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Immunology And Serology > ASO Agglutination Test > Flashcards

ASO Agglutination Test Flashcards

1
Q

ASO

A

Anti-streptolysin O

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2
Q

streptococci characteristics

A

gram positive spherical, ovoid, lancet shaped organism
catalase negative that are often seen in pairs or chains under microscope

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3
Q

what is the basis of the division of streptococci into different serogroups?

A

cell wall components

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4
Q

what major proteins are contained on the outermost cell wall components?

A

M and T protein

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5
Q

these determine the serotype or serogroup of the streptococcus spp.

A

M and T protein

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6
Q

interior to the protein layer that divides streptococci into 20 defined groups

A

group-specific carbohydrate

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7
Q

20 defined groups of streptococci

A

A-H, K-V

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8
Q

some biologically important streptococcus belong to what groups

A

group A, B, C, D

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9
Q

group of s. pyogenes

A

group A

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10
Q

group of s. galactiae

A

group B

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11
Q

group of s. dysgalactiae

A

group C

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12
Q

group of s. bovis

A

group D

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13
Q

group of enterococcus

A

group D

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14
Q

one of the most common and ubiquitous pathogenic bacteria and causes a variety of infection

A

s. pyogenes

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15
Q

diseases caused by s. pyogenes

A

scarlet fever, bacterial acute glomerulonephritis, acute rheumatic fever

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16
Q

what is the major virulence facto of s pyogenes of group A?

A

m protein

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17
Q

what is the function of m protein

A

inhibits phagocytosis by inhibiting adhesion process

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18
Q

other virulence factors of s pyogenes

A

exotoxins

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19
Q

responsible for the rash seen in scarlet fever and also appear to contribute to pathogenicity

A

pyrogenic exotoxins A, B, and C

20
Q

enzymes produced by streptococcus

A

streptolysin O, DNAse B, hyaluronidase, NADase, streptokinase

21
Q

the most diagnostically important antibodies

A

ASO, anti-DNAse B, anti-NADase, anti-hyaluronidase

22
Q

Group A streptococcus infection is seen in conditions like

A

scarlet fever, acute glomerulonephritis, acute rheumatic fever, necrotizing fasciitis, streptococcal shock syndrome

23
Q

what is the principle of ASO agglutination test

A

passive agglutination in which antigen is accompanied by a carrier particle

24
Q

what does the ASO agglutination test detect?

A

antibody

25
Q

what is the significant titer for adults in ASO agglutination test

A

240 Todd unit

26
Q

what is the significant titer for children in ASO agglutination test?

A

320 Todd units

27
Q

what is preferred sample for the ASO agglutination test?

A

patient’s serum sample

28
Q

what kind of method is ASO procedure?

A

qualitative method

29
Q

what to do if we receive a positive reaction?

A

proceed with quantitative method (serial dilution)

30
Q

PROCEDURE for ASO agglutination test

A

same as CRP

31
Q

what does the positive reaction of ASO agglutination test indicate?

A

> 200 Todd units

32
Q

what is the limit of sensitivity of ASO agglutination test?

A

200 (same as dilution factor)

33
Q

what does the negative reaction for the serial dilution indicate?

A

200 Todd units

34
Q

what is the normal level for ASO?

A

<200 Todd units

35
Q

ASO latex reagents

A

a suspension of polystyrene latex particles sensitized with streptococcal exoenzyme

36
Q

ASO positive control

A

a stabilized human serum containing ASO reactive with ASO latex reagent

37
Q

ASO negative control

A

a stabilized human serum containing ASO nonreactive with ASO latex reagent

38
Q

what is used as a reagent’s preservative?

A

0.1% sodium azide

39
Q

diagnosis of the probability for sequelae S. pyogenes infection

A

ASO tube test

40
Q

dilution used for ASO tube test

A

compound dilution

41
Q

principle of ASO tube test

A

neutralization/ enzyme inhibition test

42
Q

what is the positive reaction for ASO tube test?

A

no hemolysis

43
Q

what is the significant titer for ASO tube test?

A

166 Todd units

44
Q

antigen excess

A

prozone effect

45
Q

antibody excess

A

postzone

Immunology And Serology (11 decks)

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