CRP Determination by Agglutination reaction Flashcards

1
Q

normal serum proteins that is increase rapidly by at least 25% as a result of infection, injury, or trauma to the tissues

A

APR

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2
Q

function of CRP

A

opsonization, complement activation

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3
Q

response time of CRP

A

6-10 hours

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4
Q

function of Serum Amyloid A

A

removal of cholesterol

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5
Q

response time of Serum Amyloid A

A

24 hours

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6
Q

function of Alpha1-antitrypsin

A

protease inhibitor

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7
Q

function of fibrinogen

A

clot formation

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8
Q

function of haptoglobin

A

binds hgb

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9
Q

function of ceruloplasmin

A

binds copper and oxidizes iron

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10
Q

function of complement C3

A

opsonization, lysis

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11
Q

function of mannose-binding protein

A

complement activation

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12
Q

response time of alpha1-antitrypsin

A

24 hours

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13
Q

response time of fibrinogen

A

24 hours

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14
Q

response time of haptoglobin

A

24 hours

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15
Q

response time of ceruloplasmin

A

48-72 hours

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16
Q

response time time of complement c3

A

48-72 hours

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17
Q

where are APR produced primarily?

A

hepatocytes

18
Q

APR responds to the increase of what

19
Q

they play an important role during the activation and effector phases during an immune response

20
Q

intracellular signaling polypeptides that can influence the activities of other cells

21
Q

families of cytokines

A

IF, IL, TNF, CSF

22
Q

what are the 2 major APR?

23
Q

is APR non-specific?

24
Q

what is the principle of CRP?

A

reverse passive agglutination

25
employs particles that are coated with antibodies
reverse passive agglutination
26
what is the reagent for the determination of CRP
anti-human CRP carried by latex particle
27
what is the carrier particle in the anti0human CRP?
latex particle
28
what are we trying to detect in the determination of CRP?
antigen on px sample
29
what does the presence of visible agglutination indicate?
CRP of more than 6mg/L
30
what is used to dilute the positive sera sample?
0.85-0.90% NSS
31
PROCEDURE of CRP determination:
1. reagents and specimen at RT; mix well 2. deliver 40-50 uL of serum sample 3. add 1 drop of positive and negative control to separate ovals 4. mix well the latex reagent until the suspension is homogenous. place one drop of CRP latex reagent 5. mix each specimen and control until the entire area of each oval is filled 6. tilt the slide back and forth for 2 minutes then observe for agglutination
32
what is the procedure for CRP determination used for?
screening, qualitative test
33
what should be done if the patient sample gives a positive reaction?
perform serial dilution
34
purpose of serial dilution
determine the titer and determine the concentration of CRP in the px serum
35
how much diluent is put in each test tube?
100 uL
36
how much solute is put in each test tube?
100 uL
37
what should be done on the 6th test tube?
discard 100 uL of the dilution
38
reciprocal of the last dilution with positive reaction
titer
39
how do you determine the concentration of the titer?
titer x 6
40
what unit is used for the concentration of CRP
mg/L
41
factor that depends on the reagent used (6)
limit of sensitivity