CRP Determination by Agglutination reaction Flashcards
normal serum proteins that is increase rapidly by at least 25% as a result of infection, injury, or trauma to the tissues
APR
function of CRP
opsonization, complement activation
response time of CRP
6-10 hours
function of Serum Amyloid A
removal of cholesterol
response time of Serum Amyloid A
24 hours
function of Alpha1-antitrypsin
protease inhibitor
function of fibrinogen
clot formation
function of haptoglobin
binds hgb
function of ceruloplasmin
binds copper and oxidizes iron
function of complement C3
opsonization, lysis
function of mannose-binding protein
complement activation
response time of alpha1-antitrypsin
24 hours
response time of fibrinogen
24 hours
response time of haptoglobin
24 hours
response time of ceruloplasmin
48-72 hours
response time time of complement c3
48-72 hours
where are APR produced primarily?
hepatocytes
APR responds to the increase of what
cytokines
they play an important role during the activation and effector phases during an immune response
cytokines
intracellular signaling polypeptides that can influence the activities of other cells
cytokines
families of cytokines
IF, IL, TNF, CSF
what are the 2 major APR?
CRP, SAA
is APR non-specific?
yes
what is the principle of CRP?
reverse passive agglutination
employs particles that are coated with antibodies
reverse passive agglutination
what is the reagent for the determination of CRP
anti-human CRP carried by latex particle
what is the carrier particle in the anti0human CRP?
latex particle
what are we trying to detect in the determination of CRP?
antigen on px sample
what does the presence of visible agglutination indicate?
CRP of more than 6mg/L
what is used to dilute the positive sera sample?
0.85-0.90% NSS
PROCEDURE of CRP determination:
- reagents and specimen at RT; mix well
- deliver 40-50 uL of serum sample
- add 1 drop of positive and negative control to separate ovals
- mix well the latex reagent until the suspension is homogenous. place one drop of CRP latex reagent
- mix each specimen and control until the entire area of each oval is filled
- tilt the slide back and forth for 2 minutes then observe for agglutination
what is the procedure for CRP determination used for?
screening, qualitative test
what should be done if the patient sample gives a positive reaction?
perform serial dilution
purpose of serial dilution
determine the titer and determine the concentration of CRP in the px serum
how much diluent is put in each test tube?
100 uL
how much solute is put in each test tube?
100 uL
what should be done on the 6th test tube?
discard 100 uL of the dilution
reciprocal of the last dilution with positive reaction
titer
how do you determine the concentration of the titer?
titer x 6
what unit is used for the concentration of CRP
mg/L
factor that depends on the reagent used (6)
limit of sensitivity
