CRP Determination by Agglutination reaction

Question 1

normal serum proteins that is increase rapidly by at least 25% as a result of infection, injury, or trauma to the tissues

Answer 1

APR

Question 2

function of CRP

Answer 2

opsonization, complement activation

Question 3

response time of CRP

Answer 3

6-10 hours

Question 4

function of Serum Amyloid A

Answer 4

removal of cholesterol

Question 5

response time of Serum Amyloid A

Answer 5

24 hours

Question 6

function of Alpha1-antitrypsin

Answer 6

protease inhibitor

Question 7

function of fibrinogen

Answer 7

clot formation

Question 8

function of haptoglobin

Answer 8

binds hgb

Question 9

function of ceruloplasmin

Answer 9

binds copper and oxidizes iron

Question 10

function of complement C3

Answer 10

opsonization, lysis

Question 11

function of mannose-binding protein

Answer 11

complement activation

Question 12

response time of alpha1-antitrypsin

Answer 12

24 hours

Question 13

response time of fibrinogen

Answer 13

24 hours

Question 14

response time of haptoglobin

Answer 14

24 hours

Question 15

response time of ceruloplasmin

Answer 15

48-72 hours

Question 16

response time time of complement c3

Answer 16

48-72 hours

Question 17

where are APR produced primarily?

Answer 17

hepatocytes

Question 18

APR responds to the increase of what

Answer 18

cytokines

Question 19

they play an important role during the activation and effector phases during an immune response

Answer 19

cytokines

Question 20

intracellular signaling polypeptides that can influence the activities of other cells

Answer 20

cytokines

Question 21

families of cytokines

Answer 21

IF, IL, TNF, CSF

Question 22

what are the 2 major APR?

Answer 22

CRP, SAA

Question 23

is APR non-specific?

Answer 23

yes

Question 24

what is the principle of CRP?

Answer 24

reverse passive agglutination

Question 25

employs particles that are coated with antibodies

Answer 25

reverse passive agglutination

Question 26

what is the reagent for the determination of CRP

Answer 26

anti-human CRP carried by latex particle

Question 27

what is the carrier particle in the anti0human CRP?

Answer 27

latex particle

Question 28

what are we trying to detect in the determination of CRP?

Answer 28

antigen on px sample

Question 29

what does the presence of visible agglutination indicate?

Answer 29

CRP of more than 6mg/L

Question 30

what is used to dilute the positive sera sample?

Answer 30

0.85-0.90% NSS

Question 31

PROCEDURE of CRP determination:

Answer 31

1. reagents and specimen at RT; mix well
2. deliver 40-50 uL of serum sample
3. add 1 drop of positive and negative control to separate ovals
4. mix well the latex reagent until the suspension is homogenous. place one drop of CRP latex reagent
5. mix each specimen and control until the entire area of each oval is filled
6. tilt the slide back and forth for 2 minutes then observe for agglutination

Question 32

what is the procedure for CRP determination used for?

Answer 32

screening, qualitative test

Question 33

what should be done if the patient sample gives a positive reaction?

Answer 33

perform serial dilution

Question 34

purpose of serial dilution

Answer 34

determine the titer and determine the concentration of CRP in the px serum

Question 35

how much diluent is put in each test tube?

Answer 35

100 uL

Question 36

how much solute is put in each test tube?

Answer 36

100 uL

Question 37

what should be done on the 6th test tube?

Answer 37

discard 100 uL of the dilution

Question 38

reciprocal of the last dilution with positive reaction

Answer 38

titer

Question 39

how do you determine the concentration of the titer?

Answer 39

titer x 6

Question 40

what unit is used for the concentration of CRP

Answer 40

mg/L

Question 41

factor that depends on the reagent used (6)

Answer 41

limit of sensitivity