FA Special Stains and Cultures Flashcards
Chlamydia, Borrelia, Ricketsiae, Trypanosomes, and Plasmodium can all be visualized with what special stain?
Giemsa
Giema staining bugs - Certain Bugs Really Try My Patience
Chlamydia Borrelia Rickettsiae Trypanosomes Plasmodium
This special stain turns glycogen & mucopolysaccharides bring pink;
PAS (periodic acid-Schiff)
PAS the sugar
What bug can be detected by PAS?
Tropheryma whipplei
What bugs can be detected by Ziehl-Neelsen (carbon fuchsin)?
Acid-fast organisms (Nocardia, Mycobacterium)
What bug can be detected by India ink?
Cryptococcus
What bugs can be detected by silver stain
Fungi (eg Pneumocystic), Legionella, H pylori
What bug can be isolated with chocolate agar with factors V (NAD+) and X (hematin)?
H influenzae
What bugs can be isolated with Thayer-Martin (or VPN) media?
N gonorrhoeae, N meningitidis
To connect Neisseria, please use your VPN client
What are the three components of Thayer-Martin (VPN) culture?
Vancomycin
Polymyxin
Nystatin
Why is vanco included in VPN?
Inhibits gram positive organisms
Why is polymyxin included in VPN?
inhibits gram negs except neisseria
why is nystatin included in VPN?
inhibits fungi
What can be isolated with Tellurite plate or Loffler’s media? (These are two things)
C diphhtheriae
What can be isolated with Lowenstein-jensen agar?
M tubuerculossis
What can be isolated with Bordet-Gengous (potato) agar?
B pertussis
What can be isolated with Eaton’s agar?
M pneumoniae
What grows pink colonies on MacConkey’s agar?
Lactose-fermenting enterics
Why do lactose fermentors turn MacConkey pink?
Fermentation produces acid, turning colony pink
What grows on eosin-methylene blue (EMB) as colonies with green metallic sheen?
E coli
What grows on charcoal yeast extract agar buffered with cystein and iron?
Legionella
What grow’s on Sabouraud’s agar?
Fungi. Sab’s a fun guy!
