FA Gram Stain Taxonomy Flashcards

1
Q

What are the gram positive cocci?

A

Staph and strep

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2
Q

What are the gram negative cocci?

A

Neisseria

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3
Q

What are the gram positive rods?

A
Clostridium
Corynbacterium
Bacillus
Listeria
(Mycobacterium - acid fast)
(Gardnerella - Gram variable)
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4
Q

What are the respiratory gram negative rods?

A

Haemophilus (pleomorphic)
Legionella (silver)
Bordetella

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5
Q

What are the zoonotic gram negative rods?

A

Francisella
Brucella
Pasteurella
Bartonella

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6
Q

There are 13 enteric gram negative rods. Tell me 5. In micro, assume gram negative rod unless you have memorized it to be something else.

A
E coli
Shigella
Salmonella
Yersinia
Klebsiella
(learn rest later I guess)
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7
Q

What are the gram positive branching filamentous bacteria?

A

Actinomyces

Nocardia (weakly acid fast)

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8
Q

What are the pleomorphic gram negatives?

A

Rickettsiae (Giemsa)

Chlamydiae (Giemsa)

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9
Q

What are the spiral spirochetes (which I guess you consider to be gram negative…)?

A

Leptospira
Borrelia (Giemsa)
Treponema

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10
Q

What bacteria has a sterol-containing membrane and no cell wall?

A

Mycoplasma (gram invisible!)

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11
Q

What bacteria has a my colic acid containing membrane and high lipid content?

A

Mycobacteria

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12
Q

There are 6 bugs that do not gram stain well. These Rascals May Microscopically Lack Color

A
Treponema
Rickettsia
Mycobacteria
Mycoplasma
Legionella pneumophila
Chlamydia
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13
Q

This bug does not gram stain well because it is too thin to be visualized.

A

Treponema

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14
Q

How do you visualize Treonemes?

A

Dark-field microscopy and fluorescent antibody staining

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15
Q

What intracellular parasite can’t be gram stained?

A

Rickettsia

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16
Q

What bacteria can’t be gram stained do to the high lipid content of its cell wall?

A

Mycobacteria

17
Q

How do you detect mycobacteria and why does that work?

A

high lipid content in cell wall deterred by carbolfuchsin in ACID FAST stain

18
Q

What bacteria can’t be detected by gram stain because it has no cell wall?

A

Mycoplasma

19
Q

Why can’t you see Legionella on gram stain?

A

Primarily intracellular

20
Q

What do you use to visualize Legionella?

A

Silver stain.

21
Q

Why bacteria can’t be seen on gram stain because it is an intracellular parasite that lacks muramic acid in its cell wall?

A

Chlamydia