export_bact 5 Flashcards
grow as mononucleated single cells, pathogenic yeasts reproduce by budding (called blastospores), may produce elongated forms that resemble hyphae (pseudohyphae), colony is typically “moist”
Yeast
grow as multicellular and multinucleate filamentous colonies, reproduce by sporulation, body: hyphae (may be septate or aseptate)
Mold
occur in both mold and yeastforms
Dimorphis fungi
method of reproduction for fungi (the processof forming spores)
sporulation
the tubular, branching, multinucleatestructures that form the “body” of the mold
hyphae
hyphae having crosswalls
septate hyphae
few or no crosswalls
aseptate hyphae
a mass of interwoven hyphae
mycelium
sexual reproductive (perfect)state of fungi
Teleomorph
asexual reproductive (imperfect) state offungi
anamorph
large (usually greater than 4x12 microns)multi-celled spores, the large spores that are producedby Microsporum canis that are so characteristic that they aid in speciesidentification
Macroconidia
small (usually less than 5 microns) one-celledspores
microconidia
asexual spores produced by segmentation(fragmentation) of hyphae (characteristic of Coccidioides immitis innature and the dermatophyte in vivo), the term for thetype of fungal spores that are produced by segmentation of the hyphae
arthrospores
asexual spores produced by budding from amother yeast cell (e.g. Cryptococcus neoformans ), thetype of spores that are produced from Cryptococcus neoformans
blastospores
main component of cell wallof fungi , N-acetyl glucosamine residues, the periodic-acid-schiff stain is useful for its ability tostain this compound
chitin
D-glucose resides
glucan
cytoplasmic membrane, contains ergosterol, the name for the fungal structure that corresponds to thecytoplasmic membrane of bacteria
plasmalemma
majorsterol in cytoplasmic membranes, alters permeability, thiscompound is in the plasmalemma but not in mammalian cell structures
ergosterol
The type of spores that are produced by yeasts are called
blastospores
Dimorphic fungi have the ability to grow inthese two fungal forms
Mold & Yeast forms
Hyphae with crosswalls
Septae
Sexual reproductive ( perfect ) state offungi
teleomorph
The type of fungal spores that are produced by segmentation of thehyphae are
arthrospores
Term used in reference to Arthrospores being formedinside the hair shaft
endothrix
Term used in reference to Arthrospores beingformed outside the hair shaft
ectothrix
This compound is a structural component of thecell wall of fungi but not of bacteria
chitin
The name used for the fungal structure thatcorresponds to the cytoplasmic membrane of bacteria
plasmalemma
This compound is in the Plasmalemma but not inmammalian cell structures
ergosterol
The Plasmalemma is equivalent to what structure in bacteria
cytoplasmic membrane
The type of cell structure characteristic of the fungi
eukaryotic
toxic mushrooms belong to this class of fungi
Basidiomycetes
most members of deuteromycetes are placed in this class when their “perfect state” is delineated
Ascomycetes
this form class contains most of the fungi pathogenic to animals
Deuteromycetes ( Fungi imperfecti)
The Domain to which the fungi belong
Eukarya
The kingdom to which the fungi belong
Fungi
The toxic mushrooms belong to this class of fungi
Basidiomycetes
Most members of Deuteromycetes are placed in the class when their”perfect state” is delineated
Ascomycetes
This Form class contains most of the fungi pathogenic to animals
Deuteromyecetes
Most of the pathogenic fungi as isolated from animals have been placedin this subdivision of the fungal classification system
DEUTEROMYCOTINA(Deuteromycetes)
This agent, a common cause of “Phycomycosis”, may not be afungi but may be a member of the kingdom Protista
Pythium insidiosum
This “fungus” does not have chitin in the cell wall
Pythium insidiosum
intoxicationdue to ingestion of toxic metabolites that are produced by the action ofcertain molds in some food products
Mycotoxicosis
produce aflatoxinsthus causing aflatoxicosis, genus of fungi is one ofthe genera commonly implicated in mycotoxicosis , most common fungal species isolated from guttural pouch mycosis in thehorse Term
Aspergillus
produces aflatoxins, mostprobable exudate in a nasal discharge in a dog presenting with a nasaldischarge
Aspergillus flavus
An infection that involves the epidermal layers of skin, hair and nails
Dermatophytosis
A subcutaneous granulomatous abscess thatspreads to adjacent soft tissue, fascia and bone
Mycetoma
An intoxication due to ingestion of toxicmetabolites produced by the action of certain molds on some food products
Mycotoxicosis
These two genera of fungi are commonly implicated in mycotoxicosis
Aspergillus &Fusarium
This species of fungi is known to produce toxins which causemycotoxicosis in animals
Aspergillus fumigates
What is the major target organ in acute aflatoxin poisoning in animals?
liver
used to visualize capsulesof yeasts
India ink
best stain to visualize fungi from culture growth
Lactophenol CottonBlue
the best way to visualizethe fungal cell wall in histopathology
Periodic-Acid-Schiff (P.A.S.) stain
base media used to growfungi
Sabouraud’s Dextrose Agar
antibiotic present in Sabouraud’s DextroseAgar, inhibits nonpathogenic fungi
Cycloheximide
almost specific for isolation ofdermatophytes, contains antibiotics: cycloheximide, gentamicin, andchlortetracycline, indicator: phenol red, red color indicates alkaline
Dermatophyte test medium (DTM)
The best stain to visualize the fungal cellwall in histopathology
PAS
You have submitted a tissue specimen to a diagnostic laboratory. You request that they stain a histopathologicslide specifically for fungi. They wouldprobably use this stain
Periodic-Acid-Schiffstain
The periodic-acid-Schiff stain is useful forstaining fungi because it reacts specifically with this compound in the fungalcell wall
chitin
You are taking a skin scraping for culture ofa dermatophyte from a dog. What is therecommended incubation temperature for this specimen?
25 C
The base media usually used to grow fungi
Sabourauds Dextrose Augar
This drug is commonly added to media used togrow pathogenic fungi to keep other contaminating fungi from growing
Cycloheximide
The best stain to visualize fungi from culturegrowth
Lactophenol Cotton Blue
Used to visualize capsules of yeasts
India ink
termused by physicians to refer to a fungal infection of keratin in specific bodyareas
Tinea
termused for dermatophytes whose major source to the animal is the soil
Geophilic
humansare the maintaining host of these types of dermatophytes
Anthropophilic
the large spores that areproduced by Microsporum canis that are so characteristic thatthey aid in species identification
Macroconidia
most common cause ofdermatophytosis in the cat
Species Microsporum canis
geophilic fungusspecifically affects the keratinized skin layers with subsequent diseasedevelopment in the dog
Species Microsporum gypseum
8 month old Yorkshire-crosspigs in Georgia with large scaly lesion on his sides clinically diagnosed withdermatophytosis is most likely infected with this fungi
Species Microsporum nanum
this agent is the commoncause of skin disease involving only the dermal layers in horses
Species Trichophyton equinum
T erm used for dermatophyteswhose major source to the animal is the soil
geophilic
Humans are the maintaining host of these typesof dermatophytes
anthropophilic
Animals are the maintaining host of thesetypes of dermatophytes
zoophilic
Term used by physicians to refer to a fungalinfection of keratin in specific body areas (from PwrPt)
tinea
The large spores that are produced by Microsporum canis that areso characteristic that they aid in species identification are called
macroconidia
This dermatophyte is classified as geophilic
Microsporum gypseum
What is the pathological process responsible for the “ringworm” nature ofsome dermatophyte infections?
Lateral Growth of Fungus to avoid inflammatoryresponse
The major cause of dermatophytosis in cats
Microsporum canis
This dermatophyte is considered to be host-adapted to cats
Microsporum canis
Microsporum canis
- Symptoms: Lesions of”scaling and patching” on head and paws
- Laboratory: Direct mount of hairs in 30% KOH,arthrospores observed external to hair shaft
Microsporum canis
You have diagnosed a “ringworm” infection in a cat. The client asks about the possibility oftransfer of the infection to her or her children. You advised her that the “ringworm”in the cat, in regards to the possibility of transfer to humans, is:
a disease of highdanger to exposed individuals
This geophilic fungus specifically affects the keratinized skin layerswith subsequent disease development in the dog.
Microsporium gypseum
Most common cause of dermatophytosis in the dog
Microsporium canis
This keratinophilic fungus is host-adapted to the horse
Trichophyton equinum