EXPERIMENTAL TECHNIQUES

Question 1

WHAT EXPERIMENTAL TECHNIQUES CAN BE EMPLOYED TO FIND OUT WHERE AND WHEN A GENE IS EXPRESSED IN AN EMBRY/ WHERE AND WHEN A GENE IS EXPRESSED

Answer 1

1. IN SITU HYBRIDIZATION
2. NORTHERN BLOT
3. RT -PCR
4. MICROARRY
5. REPORTER LINES

Question 2

WHAT EXPERIMENTAL TECHNIQUES MIGHT BE USED TO SEE IF A PROTEIN IS EXPRESSED WITH THE SAME TIMING AS THE GENE YOU ARE LOOKING AT/ WHAT IS THE DISTRIBUTION OF PROTEINS

Answer 2

1. WESTERN BLOT
2. IMMUNOHISTOCHEMISTRY
3. IMMUNOFLUORESCENCE

Question 3

WHAT EXPERIMENTAL TECHNIQUES MIGHT YOU EMPLOY TO SEE IF A GENE OR PROTEIN IS ESSENTIAL FOR DEVELOPMENT
/ DETERMINE THE FUNCTION OF A GENE

Answer 3

GOF
LOF
FORWARD/REVERSE GENETICS

Question 4

WHAT EXPERIMENTAL TECHNIQUES MIGHT YOU EMPLOY TO SEE HOW A GENE IS REGULATED
/ DEMONSTRATE INDUCTIVE FUNCTION

Answer 4

EMBRYOLOGY: TISSUE MANIPULATION (GRAFT,ABLATION)

MANIPULATING SIGNALLING PATHWAYS: DRUGS,ELECTROPORATION, PROTEIN SOAKED BEADS

Question 5

WHAT EXPERIMENTAL TECHNIQUES MIGHT YOU EMPLOY TO SEE WHAT TISSUES/ORGANS ARE DERIVED FROM THE CELLS THAT EXPRESS A GENE

/FATE MAPS AND LINEAGE ANALYSES

Answer 5

EMBRYOLOGY: CHICK/QUAIL CHIMERA, DYE LABELLING

GENETICS: LABELLING WITH RETROVIRUS, GFP