Exam Revision - From Notes Flashcards
The dependent variable is the variable we __________
measure
The independent variable is the variable we __________
change
A controlled variable is also known as the
Constant variable
Types of sampling
- Systematic sampling
- Random sampling
- Stratified sampling
- Judgement sampling
- Convenience sampling
Types of errors
- Personal error
- Systematic error
- Random error
Primary data is when you’re collecting _________ data
your
Raw data vs Transformed data
Raw data is as you write the results down in your logbook.
Transformed data is when you start graphing it or presenting it in tables.
Two types of variables
- Numerical
- Categorical
Two types of numerical data
- Discrete
- Continuous
Two types of categorical data
- Ordinal data
- Nominal data
Bar graphs are typically used to display ____________ data
Categorical and discrete
Line graphs and scatter plots display __________________ data
Continuous
A question to assess precision
are the results within the replicate treatments similar or different?
A question to assess accuracy
Are the recorded values similar or different from the true values?
A question to assess outliers
Are there any data points that stand out or do not follow the pattern?
Three approaches to bioethics
consequence based approach, rule/duty based approach, and virtues-based approach.
What is the aim of a consequence-based approach?
to maximise positive outcomes whilst minimising negative effects.
What is the aim of a rules/duty based approach?
To follow a set of rules and responsibilities, with less regard of the consequences as a result.
What is the aim of a virtues-based approach?
To emphasise the moral nature of the individual, and provide guidance as to the behaviours a morally good person would hope to achieve.
The five ethical concepts in Biology
integrity, justice, beneficence, nonmaleficence, and respect.
Integrity is the commitment to _____________-
Knowledge
Justice is the commitment to ________________
Fairness
Beneficence is the commitment to ____________
Maximising benefits
Nonmaleficence is the commitment to ____________________
Minimising harm
Respect is the commitment to __________________
Consideration
What are 8 qualities that all living things possess?
Movement
Respiration
Sensitivity
Growth
Reproduction
Equilibrium
Excretion
Nutrition.
What does cell theory state?
- All living things are made up of cells.
- Cells are the smallest and most basic units of life.
- All cells come from pre-existing cells.
What are the Six Kingdoms of life
Animalia, Archaea, Bacteria, Fungi, Plantae, and Protista
How do eukaryotic cells replicate?
Mitosis and meiosis
How do prokaryotic cells replicate?
binary fission
Differences between eukaryotic and prokaryotic cells.
Eukaryotic cells have membrane-bound organelles whilst prokaryotes do not
Eukaryotic cells have multiple linear strands of DNA packaged in chromosomes in the nucleus whilst prokaryotes have one circular chromosome and some additional plasmids.
What are the three domains of life?
- Archaea
- Bacteria
- Eukarya
What domains are prokaryotic, and which is eukaryotic?
Prokaryotic: Archaea and bacteria
Eukaryotes: Eukarya
What kingdoms can Eukarya be divided into?
Animalia, Fungi, Plantae, and Protista.
What is an organelle?
A cellular structure that performs specific functions
What is cytosol? What is cytoplasm?
Cytoplasm is everything inside the cell except the nucleus, whilst the cytosol is the internal liquid.
What does the nucleus store?
Genetic material
The nucleus is surrounded by a _______________________
double membrane
What does the nucleolus produce?
Ribosomes
What are ribosomes made up of?
Ribosomal RNA
Ribosomal RNA can be abbreviated to?
rRNA
Where are two places ribosomes can be found in the cell?
They can float freely in the cytoplasm or are attached to the rough ER
Ribosomes assemble the building blocks to build _______________
proteins
The Rough ER is coated with ___________________
ribosomes
What does the Rough ER do?
It synthesises and modifies proteins
Smooth ER are responsible for the production of _______________
lipids
The Golgi Apparatus is also known as the _________________
Golgi body
The Golgi Apparatus does what?
Packages and modifies proteins readying them for cell use or export
Lysosomes contain ________________________
Digestive enzymes
What do lysosomes do?
Break down cell waste and toxins.
What does the Mitochondria do?
the site of aerobic respiration cellular respiration and produces ATP.
Chloroplasts are the site of ___________________
photosynthesis
Vacuole is used for ________
storage
The vacuole helps maintain _____________ in ___________ cells.
structure, plant
What type of cells are the cell wall found?
Plant, bacterial, and fungal cells
Which organelles are not membrane bound?
Ribosomes, cell wall, and cytoskeleton
The folds of the inner membrane of the mitochondria are known as the
cristae
The space inside the inner membrane is known as the
Mitochondrial matrix
The word formula for aerobic cellular respiration is
glucose + oxygen = carbon dioxide + water + energy.
The chemical formula for aerobic cellular respiration is
C6H12O6 + 6O2 = 6CO2+ 6H2O + 36 ATP
What kind of cells can chloroplasts be found in?
Plant and algae cells
The chemical formula for photosynthesis is
6CO2 + 6H2O = C6H12O6 + 6O2
The word formula for photosynthesis is
Carbon Dioxide + Water = Glucose + Oxygen
What are some of the key differences between plant and animal cells.
The key differences between animal and plant cells is that plant cells have a cell wall whilst animal cells do not, chloroplasts are present in plant cells but not in animal cells, and vacuoles in animal cells are small and there can be many or none whilst plant cells tend to have one large vacuole.
What are the benefits of having small cells?
(1) The exchange of materials with the extracellular environment can occur efficiently (2) Distances to travel within the cell are smaller, so intracellular transport is faster.
What does the plasma membrane separate?
the intracellular environment to the extracellular environment
The main component of the phospholipid bilayer are
Phospholipids
What is the name of the arrangement of phospholipids?
The phospholipid bilayer
What are the two components of phospholipids
- Phosphate head
- Fatty acid tails
What is the phosphate head made up of?
Glycerol and phosphate group
What is the charge of the phosphate head?
Negatively charged
Is the phosphate head hydrophobic or hydrophilic?
Hydrophilic
Is the phosphate head polar or non polar?
Polar
What are the two fatty acid tails made up of?
Chains of Carbon and Hydrogen
What is the charge of the two fatty acid tails?
Uncharged
Are the two fatty acid tails hydrophobic or hydrophilic?
Hydrophobic
Are the two fatty acid tails polar or non polar?
Non polar
As phospholipids have both hydrophobic and hydrophilic parts they are ____________________ molecules
amphipathic
What are the names of the three types of proteins embedded in the plasma membrane?
- Integral proteins
- Transmembrane proteins
- Peripheral proteins
What are integral proteins?
proteins that are a permanent part of the membrane
What are transmembrane proteins?
integral proteins that span the entire bilayer
What are peripheral proteins?
are temporarily attached to the plasma membrane
What are the four functions/purposes of proteins in the plasma membrane?
- Transport
- Catalysis
- Communication
- Adhesion
Where is cholesterol in the plasma membrane?
Between the fatty acid tails
What are the two types of carbohydrates present in the membrane?
- Glycolipids
- Glycoproteins
What are glycolipids
Carbohydrates rooted to lipids
What are glycoproteins?
Carbohydrates rooted to proteins
What three functions do carbohydrates perform?
cell-cell communication, signaling, and adhesion
What does cholesterol regulate in the plasma membrane?
Fluidity
What 2 things does the fluid mosaic model dictate?
1) molecules that make up the membrane are not held static in one place and 2) many different types of molecules are embedded in the plasma membrane.
Active transport requires ________________
energy
What are the two types of active transport
- Protein mediated transport (or just “Active Transport”)
- Bulk transport
What are the two requirements for when a molecule needs to be transported against its concentration gradient?
- Energy
- Membrane proteins
How is bulk transport different from protein mediated transport?
Protein-mediated transport uses membrane proteins whilst bulk transport uses vesicles
What are the three steps of protein mediated transport?
- Binding
- Conformational change
- Release
What reaction occurs during conformational change to release energy?
ATP —> ADP + P
What are two types of bulk transport?
- Endocytosis
- Exocytosis
What is the difference between endocytosis and exocytosis?
Exocytosis is for molecules exiting the cell and endocytosis is for molecules entering the cell.
Steps of exocytosis
- Vesicular transport
- Fusion
- Release
Steps of endocytosis
- Fold
- Trap
- Bud
What is phagocytosis?
The endocytosis of solid materials - ‘cell eating’
What is pinocytosis?
The endocytosis of liquid materials - ‘cell drinking’
What are the purposes of cell replication?
1) Growth and development, 2) Maintenance and repair, 3) Reproduction
Prokaryotes replicate ex…….
ponentially
What is the process called through which prokaryotes replicate?
Binary fission
Binary fission is a form of ________________ reproduction
asexual
Steps of binary fission
- DNA replication
- Elongation
- Septum formation
- Cell division
What are the three stages of the eukaryotic cell cycle?
Interphase, mitosis, cytokinesis
What is the longest stage of eukaryotic cell division?
Interphase
Three stages of interphase
- G1
- S
- G2
What occurs during G1?
the volume of cytosol increases, proteins get synthesised, and organelles replicate
What occurs during the S phase?
the cell replicates its DNA by creating sister chromatids.
Sister chromatids are held together by a centromere
Why do cells go to the G0 stage?
As they are no longer required to replicate
What are the two types of cells that go to G0?
- Quiescent cells
- Terminally differentiated cells
Can quiescent cells and/or terminally differentiated cells re-enter the cell cycle?
Quiescent cells can re-enter
Terminally differentiated cells cannot
What happens in G2?
there is an increase in the volume of cytosol, and protein synthesis occurs.
What are the four stages of mitosis?
- Prophase
- Metaphase
- Anaphase
- Telophase
What occurs during prophase?
chromosomes condense, spindle fibres form, centrioles migrate to the poles of the cell, the nuclear membrane breaks down, and the nucleolus disappears.
What occurs during metaphase?
spindle fibres fully form, and chromosomes line up at the equator of the cell.
What occurs during anaphase?
the chromatids go to opposite ends of the cell.
What occurs during telophase?
Spindle fibres disintegrate, chromosomes decondense, nuclear membrane is formed, and two genetically identical nuclei get produced.
What occurs during cytokinesis?
cytoplasm divides and organelles evenly distribute themselves.
What is different between how animal and plant cells undergo cytokinesis?
In animal cells, during cytokinesis, a cleavage furrow develops which pinches the cell in half whilst in plant cells, a cell plate forms at the equator before the cell splits in two.
How many checkpoints has the cell cycle got?
3
What are the 3 checkpoints of the eukaryotic cell cycle?
- G1 checkpoint
- G2 checkpoint
- Metaphase checkpoint
What does the G1 checkpoint verify?
the cell has grown to the correct size, and has synthesised enough protein for DNA replication.
What does the G2 checkpoint verify?
The G2 checkpoint ensures that DNA has been replicated properly and that the cell has enough resources for mitosis
What does the metaphase checkpoint verify?
the cell checks the formation of the spindle fibres and if the chromosomes are in the right location.
What is apoptosis?
The controlled and systematic death of cells, programmed cell death
What are the two pathways of apoptosis?
- Mitochondrial pathway
- Death receptor pathway
Steps of the mitochondrial pathway
- Mitochondria detect cell malfunction
- Mitochondria release cytochrome c into the cytosol
- Caspase enzyme activated
- Apoptosis begins
Steps of the death receptor pathway
- Death receptor proteins recognise death signalling molecules
- molecules bind with the receptor causing the initiation of the caspase enzyme
- apoptosis is initiated
What is necrosis?
the unregulated and uncontrolled death of cells which causes cells to swell and burst.
What are the consequences to necrosis?
may lead to inflammation and damage to nearby cells and tissues.
Steps of apoptosis
- Activate of caspase enzyme
- digestion of cell contents, 3. cell shrinkage,
- membrane blebbing.
What occurs after apoptosis?
phagocytosis
What happens if the cell cycle is disrupted?
damaged cells will replicate exponentially leading to the development of cancer and tumours
What are the two categories of tumours?
benign tumours and malignant tumours
What is the difference between benign tumours and malignant tumours?
malignant tumours can invade nearby tissue and enter the blood spread and spread
Are benign or malignant tumours cancerous?
Malignant = cancerous
Benign = not cancerous