Exam 3 Review Flashcards

0
Q

Glycolysis Step 2

A

G6P –> F6P

phosphoglucose isomerase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
1
Q

Glycolysis Step 1

A

glucose –> G6P
Hexokinase
Irreversible, USE 1 ATP

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Glycolysis Step 3

A

F6P –> F-1,6-BP
PFK 1
Irreversible, USE 1 ATP

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Glycolysis Step 4

A

F-1,6-BP –> glyceraldehyde 3-P

Aldolase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Glycolysis Step 5

A

glyceraldehyde 3-P –> DHP

triose phosphate isomerase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Glycolysis Step 6

A

glyceraldehyde 3-P –> 1,3-BPG
“ “ dehydrogenase
NAD+ –> NADH

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Glycolysis Step 7

A

1,3-BPG –> 3-PG

phosphoglycerate kinase, GAIN 1 ATP

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Glycolysis Step 8

A

3-PG –> 2-PG

Phosphoglycerate mutase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Glycolysis Step 9

A

2PG –> phosphoenolpyruvate

enolase, lose H20

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Glycolysis Step 10

A

phosphoenolpyruvate –> pyruvate
PKA, GAIN 1 ATP
irrerversible

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What regulates pdh in high energy charge (-)?

A

high NADH, high ATP, high acetyl CoA

pyruvate will NOT continue to CAC

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

what regulates PDH low energy charge (+)

A

high pyruvate, high ADP will allow pyruvate to enter CAC

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

isocitrate dehydrogenase regulation (+) and (-)

A

+ high ADP, allosterically stimulates

- high ATP, NADH inhibits

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

alpha-ketoglutarate dehydrogenase regulation -

A
  • high ATP, and allosterically inhibited by its products, NADH and succinyl CoA
  • similar to PDH
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

what does a competitive inhibitor do and how does it affect Vmax and Km?

A

binds to active site and blocks substrate
Vmax unchanged
Km increases

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What does an uncompetitive inhibitor do and how does it affect Vmax and Km?

A

binds to adjacent site after substrate binding (ES –> ESI)
Vmax and Km changed but slope of lineweaver-burke is unchanged
Km lowers as the concentration of inhibitor increases

16
Q

What does a non-competitive inhibitor do and how does it affect Vmax and Km?

A

binds allosterically to E and/or ES complex
Vmax is decreased and cannot be obtained
Km is unchanged

17
Q

zymogen

A

inactive protease

18
Q

enteropeptidase

A

cleaves to activate zymogen

19
Q

what enzyme is synthesized in stomach?

A

pepsin

20
Q

what enzymes are synthesized in the pancreas?

A

chymotrypsin, trypsin, procarboxypeptidase, proelastase