Exam 3 Review Flashcards
Glycolysis Step 2
G6P –> F6P
phosphoglucose isomerase
Glycolysis Step 1
glucose –> G6P
Hexokinase
Irreversible, USE 1 ATP
Glycolysis Step 3
F6P –> F-1,6-BP
PFK 1
Irreversible, USE 1 ATP
Glycolysis Step 4
F-1,6-BP –> glyceraldehyde 3-P
Aldolase
Glycolysis Step 5
glyceraldehyde 3-P –> DHP
triose phosphate isomerase
Glycolysis Step 6
glyceraldehyde 3-P –> 1,3-BPG
“ “ dehydrogenase
NAD+ –> NADH
Glycolysis Step 7
1,3-BPG –> 3-PG
phosphoglycerate kinase, GAIN 1 ATP
Glycolysis Step 8
3-PG –> 2-PG
Phosphoglycerate mutase
Glycolysis Step 9
2PG –> phosphoenolpyruvate
enolase, lose H20
Glycolysis Step 10
phosphoenolpyruvate –> pyruvate
PKA, GAIN 1 ATP
irrerversible
What regulates pdh in high energy charge (-)?
high NADH, high ATP, high acetyl CoA
pyruvate will NOT continue to CAC
what regulates PDH low energy charge (+)
high pyruvate, high ADP will allow pyruvate to enter CAC
isocitrate dehydrogenase regulation (+) and (-)
+ high ADP, allosterically stimulates
- high ATP, NADH inhibits
alpha-ketoglutarate dehydrogenase regulation -
- high ATP, and allosterically inhibited by its products, NADH and succinyl CoA
- similar to PDH
what does a competitive inhibitor do and how does it affect Vmax and Km?
binds to active site and blocks substrate
Vmax unchanged
Km increases