Exam 3 Flashcards
What are constitutive genes?
These are housekeeping genes and they are NOT differentially regulated, meaning they are active all the time and they ALWAYS need to be expressed in order for the cell to be alive; example: metabolism genes.
difference between housekeeping genes and specific genes.
Housekeeping genes present in all tissue types – regulate processes that all cells need (think metabolism). Specific genes are specific to their tissue type
When does the majority of gene regulation take place?
Transcription
What is an operon?
An operon is a cluster of genes that are transcribed and translated together with one promoter; only prokaryotes have them
Why are operons helpful
Because prokaryotes do not want to waste all their energy transcribing genes that they do not need to have on ALL the time so they have operons that regulate it.
What repressible operon did we learn about?
Trp operon
What is a repressor?
Binds to the operator of the operon to prevent the transcription of the genes
What is the corepressor in the trp operon?
Tryptophan
If there is tryptophan present in the cell, is the gene for tryptophan being transcribed?
No, because there is tryptophan (corepressor) that binds to the repressor and causes a shape change to activate it to bind to the operator therefore hindering transcription
What inducible operon did we learn about?
The lac operon codes for genes that make the enzymes needed to metabolize lactose a type of sugar. when the inducer binds to the repressor, the repressor is removed which allows the cell to transcribe the genes needed to make the proteins that can digest lactose.
What is the inducer in the lactose example?
Allolactose
Is the repressor normally on or off? (in lac example)
On
If there is glucose absent and lactose present is the lac operon on or off?
On
If there is glucose present and lactose present is the lac operon on or off?
Off
What type of gene regulation are operons?
Transcription
What is gene imprinting:
a process in genome modification where some genes are shut down (methylated)
What impact does DNA methylation have on transcription?
Methylation will tighten the DNA onto the histone inhibiting transcription
What impact does DNA acetylation have on transcription?
Acetylation will loosen the DNA and make transcription more likely
What are the two regulatory transcription factors?
Activators and Repressors
Activators and repressors;
activators enhance and repressors silence
Alternative splicing
*Allows for there to be many genes encoded in one transcript
*By changing what exons are included in the mRNA transcript it can change what genes are expressed and also maximize the number of genes included in one transcript
Constitutive exons:
exons always included and in the order in which they appear on the pre mRNA
Eurkaryotes
Alternative exons;
sometimes are included but other times are not always included
Eukaryotes
what is the advantage of alternative splicing
we can produce tissue specific proteins and developmental specific versions of proteins
What is quorum sensing
*Quorum sensing is the ability for bacteria to communicate with each other for the purpose of producing something; it is a way for them to alter gene expression
*The bacteria are always producing signals but once the concentration of them is high enough they will alter gene expression for the purpose of producing something
what three things can a bacteria do if it is able to communicate with other bacteria?
They can produce biofilms, toxins, and spores, exchange genetic data
What stage in the cell cycle does quorum sensing occur and therefore the things that are produced via quorum sensing?
Stationary
Examples of biofilms in real life.
Teeth, growth in catheters, growth in pipes can cause chronic infection
Why do people with diabetes usually have trouble with their feet?
Biofilms protect from antibiotics.
But the antibiotics already have a hard time circulating to the feet so the biofilm provides an extra layer therefore prolonging the infection
endotoxins
*not actively secreted and have general symptoms of fever, lethargy, malaise; produced by the LPS of gram negative bacteria
*large doses can cause hemorrhagic shock and tissue necrosis
PRODUCED BY LIPID POLYSACCHARIDES OF GRAM-
Exotoxins
secreted and have very specific targets
AB toxins;
two component exotoxins where the A component is the active toxin and the B component helps the toxin to bind to the target cell
Photoautotroph;
energy from the sun and makes its own C-C bonds
Photoheterotroph;
energy from the sun and ingests pre existing C-C bonds
Chemoautotroph;
energy from chemical bonds and makes its own C-C bonds
Chemoheterotroph;
energy from chemical bonds and ingests pre existing C-C bonds
lithotrophy
Ability to use inorganic molecules to generate energy and build cells; many are chemoautotrophs
example of lithotrophy w nitrogen cycle
ammonia oxidizers which use ammonia as a source of energy and they are critical for the nitrogen cycle to progress by oxidizing ammonia
Relationship between bacteria and root nodules.
All living things need lots of nitrogen but the only organisms that can fix it are bacteria and archaea; so plants (eukaryotes) have a mutualistic relationship with bacteria and archaea;
What type of process is nitrogen fixation
anaerobic respiration
2 plant systems
Shoot system, root system
What is the function of roots? Why do roots have root hairs?
Anchor the plant, absorb nutrients and water, store carbohydrates