Exam 2 Flashcards
Chapters 7,16
the specificity of the PCR reactions is determined by
primers
what steps can be taken for Mispriming in a PCR procedure
seclude the polymerase from the template and primers before amplification
complementarity of primer pairs at the three prime and will result in what artifacts
primer dimers
a DNA sequence with high G C content is more likely to have what property that interferes with PCR amplification
secondary structure
what type of PCR starts with a RNA template
reverse transcriptase
what is the advantage of a nested PCR procedure
nested PCR offers increase specificity and yield of product
taq-Man
a qpcr probe system
real-time PCR differs from standard PCR how
real-time PCR is quantitative
amplification control in PCR
to distinguish true negatives from false negatives
sybr green in quantitative PCR does what
detection of the PCR product
signal amplification
branched DNA assay
sybr green fluorescents is detectable at which stage of the PCR amplification
primer annealing and extension
a nucleic acid amplification procedure that hybridizes nucleic acid labeled with detectable signals
hybrid capture essay
modification of PCR uses short primers with random sequences that generate amplification of many different products
arbitrarily primed
taqman, molecular beacons, scorpion type primers are all used in what procedure
quantitative PCR
