Chapter 10

Question 1

what sequencing is performed by Chain breakage at specific nucleotides

Answer 1

maxam Gilbert

Question 2

in what direction does maxam Gilbert sequencing read

Answer 2

5 Prime to 3 Prime bottom to top

Question 3

a modified DNA replication reaction that has Growing chains that are terminated by Didioxynucleotides

Answer 3

chain termination (sanger) sequencing

Question 4

the collection of fragments is a

Answer 4

sequencing ladder

Question 5

a chain termination sequencing performed in a thermal cycler that requires a heat stable DNA polymerase

Answer 5

cycle sequencing

Question 6

multi cyclic molecules that absorb and emit fluorescent light at specific wavelengths

Answer 6

fluorescent dyes

Question 7

primer contains fluorescent dye conjugated nucleotides, labeling the sequencing ladder at the five Prime end of the chains

Answer 7

dye primer

Question 8

the fluorescent dye molecules are covalently attached to the dideoxynucleotides, labeling the sequencing ladder at the 3 Prime end of the chains

Answer 8

dye Terminator

Question 9

pyrosequencing is based on the generation of light signal through release of what on nucleotide addition

Answer 9

pyrophosphate

Question 10

pyrophosphate is used to generate ATP from what

Answer 10

adenosine phosphosulfate (APS)

Question 11

Bisulfite sequencing is used to detect what in DNA

Answer 11

methylation

Question 12

Bisulfitebisulfite deaminates cytosine, making

Answer 12

uracil