Exam 1 Flashcards
Chapters 1-6
melting temperature of nucleic acid is the temperature when
half of the double-stranded DNA is single stranded
what method is best suited for qualitative analysis of DNA such as mutational analysis
slot blot
what method is for the entire Human Genome that can be analyzed at one time
dot blot
the micro air or chip is an example of which of the following blotting procedures
reverse. dot blot
molecule added to an electrophoresis buffer for the purpose of denaturing DNA
formamide
what determines the specificity in terms of the gene that is detected in the southern blot
labeled probe
condition that is least stringent
40% formamide, 1X SSC salt at 50 degrees Celsius
what molecule polymerized to form a support medium through which nucleic acids move
polyacrylamide
what is not an example of pulse field gel electrophoresis
polyacrylamide gel electrophoresis
what electrophoresis will separate a large base pair in size mixture
rotating gel electrophoresis
what molecule will migrate the fastest in capillary electrophoresis
small and negatively charged
isolated DNA at 1.2
reprecipitate the DNA to remove excess protein
a sequence of DNA in a gene that codes for a protein is what kind of sequence
structural
in organic DNA extraction methods, the DNA is found in which
upper aqueous
diethyl pyrocarbonate is a chemical that is used to
inactivate RNAses
Polly T oligomers found to a matrix resin column will selectively isolate
messenger RNA
DNA quality can be assessed relatively simply and quickly by which of the following
electrophoresis
the sequence of amino acids in a protein constitutes which level of protein structure
primary
what describes the general structure of an amino acid
Central carbon with carboxyl group amino group hydrogen and a side chain
all but two amino acids are encoded by more than one codon thus making the genetic code
redundant
specialized proteins that bind to the large ribosomal subunit and protect the hydrophobic regions of growing polypeptides are called
molecular chaperones
DNA sequences that are involved in the regulation of gene expression
CIS factors
genomic imprinting
methylation
when gene expression is regulated by mechanisms other than the interaction of CIS elements and trans factors
epigenetics
RNA and DNA are structurally similar because they both
are polymers of four different nucleotide bases
nucleotides in a single strand of DNA are held together by
phosphodiester bonds
nucleotides in DNA consist of
nitrogen base deoxyribose and phosphate
movement of DNA from one bacterium to another through activity of bacteriophages
transduction
messenger RNA is different from other types of RNA because
messenger RNA has a poly a tail 3 Prime
restriction endonucleases are enzymes that are produced by bacteria and
degrade DNA
one DNA strand binds to the other strand through
hydrogen bonds
