Exam 1

Question 1

melting temperature of nucleic acid is the temperature when

Answer 1

half of the double-stranded DNA is single stranded

Question 2

what method is best suited for qualitative analysis of DNA such as mutational analysis

Answer 2

slot blot

Question 3

what method is for the entire Human Genome that can be analyzed at one time

Answer 3

dot blot

Question 4

the micro air or chip is an example of which of the following blotting procedures

Answer 4

reverse. dot blot

Question 5

molecule added to an electrophoresis buffer for the purpose of denaturing DNA

Answer 5

formamide

Question 6

what determines the specificity in terms of the gene that is detected in the southern blot

Answer 6

labeled probe

Question 7

condition that is least stringent

Answer 7

40% formamide, 1X SSC salt at 50 degrees Celsius

Question 8

what molecule polymerized to form a support medium through which nucleic acids move

Answer 8

polyacrylamide

Question 9

what is not an example of pulse field gel electrophoresis

Answer 9

polyacrylamide gel electrophoresis

Question 10

what electrophoresis will separate a large base pair in size mixture

Answer 10

rotating gel electrophoresis

Question 11

what molecule will migrate the fastest in capillary electrophoresis

Answer 11

small and negatively charged

Question 12

isolated DNA at 1.2

Answer 12

reprecipitate the DNA to remove excess protein

Question 13

a sequence of DNA in a gene that codes for a protein is what kind of sequence

Answer 13

structural

Question 14

in organic DNA extraction methods, the DNA is found in which

Answer 14

upper aqueous

Question 15

diethyl pyrocarbonate is a chemical that is used to

Answer 15

inactivate RNAses

Question 16

Polly T oligomers found to a matrix resin column will selectively isolate

Answer 16

messenger RNA

Question 17

DNA quality can be assessed relatively simply and quickly by which of the following

Answer 17

electrophoresis

Question 18

the sequence of amino acids in a protein constitutes which level of protein structure

Answer 18

primary

Question 19

what describes the general structure of an amino acid

Answer 19

Central carbon with carboxyl group amino group hydrogen and a side chain

Question 20

all but two amino acids are encoded by more than one codon thus making the genetic code

Answer 20

redundant

Question 21

specialized proteins that bind to the large ribosomal subunit and protect the hydrophobic regions of growing polypeptides are called

Answer 21

molecular chaperones

Question 22

DNA sequences that are involved in the regulation of gene expression

Answer 22

CIS factors

Question 23

genomic imprinting

Answer 23

methylation

Question 24

when gene expression is regulated by mechanisms other than the interaction of CIS elements and trans factors

Answer 24

epigenetics

Question 25

RNA and DNA are structurally similar because they both

Answer 25

are polymers of four different nucleotide bases

Question 26

nucleotides in a single strand of DNA are held together by

Answer 26

phosphodiester bonds

Question 27

nucleotides in DNA consist of

Answer 27

nitrogen base deoxyribose and phosphate

Question 28

movement of DNA from one bacterium to another through activity of bacteriophages

Answer 28

transduction

Question 29

messenger RNA is different from other types of RNA because

Answer 29

messenger RNA has a poly a tail 3 Prime

Question 30

restriction endonucleases are enzymes that are produced by bacteria and

Answer 30

degrade DNA

Question 31

one DNA strand binds to the other strand through

Answer 31

hydrogen bonds