Chapters 1-6

Question 1

one DNA strand binds to the other strand through

Answer 1

hydrogen bonds

Question 2

movement of DNA from one bacterium to another

Answer 2

transduction

Question 3

mRNA is different from other types of RNA because mRNA has

Answer 3

3’ poly A tails

Question 4

genomic imprinting

Answer 4

methylation

Question 5

gene expression regulated by mechanisms other than interaction of CIS elements and trans factors

Answer 5

epigenetics

Question 6

sequence of amino acids in protein constituents with which level of protein

Answer 6

primary

Question 7

what consists of a central carbon with carboxyl group, hydrogen bond, and a sidechain

Answer 7

Amino Acids

Question 8

all but two amino acids are encoded by more than one codon

Answer 8

redundant

Question 9

proteins bind to large ribosomal subunit and protect hydrophobic region of growing polypeptides

Answer 9

molecular chaperones

Question 10

sequence of DNA and Gene that codes for protein

Answer 10

structural

Question 11

nucleic acids migrate toward which pole of electrophoresis

Answer 11

anode

Question 12

what migrat faster and capillary electrophoresis

Answer 12

small and negative charged

Question 13

Southern blot is detected by

Answer 13

labeled probe

Question 14

what is the least stringent

Answer 14

40% formamide, 1X SSC, 50 degrees Celsius

Question 15

what blot is where the entire genome is

Answer 15

dot blot

Question 16

restriction endonucleases are enzymes that are produced by bacteria and

Answer 16

degrade DNA

Question 17

separating two DNA strands into two single strands

Answer 17

denaturation

Question 18

nucleotides in DNA consist of

Answer 18

nitrogen base, deoxyribose, and phosphate

Question 19

DNA sequence that regulates the gene expression

Answer 19

CIS Factor

Question 20

what are polymers of for a different nucleotide bases

Answer 20

DNA and RNA

Question 21

loses tertiary structure

Answer 21

denatured

Question 22

inorganic DNA extraction methods, the DNA is found in which fraction

Answer 22

upper aqueous

Question 23

DEPC is a chemical that is used to do what

Answer 23

inactivate RNAses

Question 24

field aversion, Contour clamping, transverse altering field electrophoresis

Answer 24

PFGE

Question 25

polymerize to form support medium through nucleic acids move

Answer 25

polyacrylamide

Question 26

what is the central dogma

Answer 26

DNA - Transcription - RNA (RNA polymerase) - Translation - Protein (peptides transferease)

Question 27

nucleic acid concentration can be assessed relatively simply and quickly by using what procedures

Answer 27

electrophoresis

Question 28

Poly T oligomers bound to a matrix resin column will selectively isolate what

Answer 28

messenger RNA

Question 29

what will not inhibit RNAases

Answer 29

Tris buffers

Question 30

the most abundant form of RNA in all cells is what

Answer 30

ribosomal

Question 31

which fixative is the least a damaging to nucleic acids

Answer 31

buffered formalin

Question 32

what is used to optimize yield of nucleic acid by precipitation or column purification

Answer 32

carrier molecules

Question 33

DNA, after separation from other cellular constituents, is precipitated with what

Answer 33

isopropanol

Question 34

fennel / chloroform and an aqueous solution will form what type of mixture

Answer 34

emulsion

Question 35

procedure used for the separation of very large nucleic acid molecules

Answer 35

rotating field gel electrophoresis

Question 36

method for the quantification of DNA sequences and mapping

Answer 36

slot blot

Question 37

DNA microarrays operate by similar procedures to those used in

Answer 37

reverse northeren dot blot