Chapter 16

Question 1

how should a DNA sample to be stored for at least 6 months be isolated

Answer 1

store at -70 degrees C in a tightly sealed tube

Question 2

what error is the consequence of erroneous or misleading results caused by events that occur prior to sample analysis

Answer 2

pre-analytical error

Question 3

precautions that include respirators for Airborne or contact transmissible agent

Answer 3

transmission based precautions

Question 4

what precautions are designed for direct patient care

Answer 4

contact precautions

Question 5

isolated RNA should be stored for less than 30 days in what

Answer 5

DEPC treated water

Question 6

RNA blood, bone marrow, fluids should be stored for less than two weeks in what

Answer 6

a denaturant

Question 7

what administration requires validation of the performance of clinical test methods and regions in accurately detecting or measuring analytes prior to use in human testing

Answer 7

FDA

Question 8

what institute documents an established procedure

Answer 8

CLSI

Question 9

what testing refers to external specimens from a reference source supplied to Independent Laboratories

Answer 9

proficiency testing

Question 10

what uses a sample of known type or amount that are treated like and run with patient specimens

Answer 10

controls

Question 11

what controls are required to avoid false negative results

Answer 11

amplification controls

Question 12

what has been shown to inhibit enzymes used in molecular analysis such as reverse transcriptase and DNA polymerase in vitro

Answer 12

Heparin and ACD

Question 13

how are solid tissues best analyzed

Answer 13

from fresh or frozen samples

Question 14

what inhibits enzyme activity

Answer 14

hemoglobin

Question 15

what tubes are used for stabilization of nucleic acids for molecular testing

Answer 15

plasma preparation tubes PPT

Question 16

what tube has sodium Heparin and tests for cytogenetic studies and molecular studies

Answer 16

Brown

Question 17

what method requires intact cellular structures or culturing of cells

Answer 17

FISH

Question 18

what tubes are for long-term nucleic acid storage at ultra low temperatures

Answer 18

cryotubes

Question 19

room temperature storage is recommended for

Answer 19

viral RNA in whole blood

Question 20

Refrigeration of blood will cause what

Answer 20

neutrophils to degranulate, releasing enzymes that affect free virus particles

Question 21

true positive and true negative are determined using what standard

Answer 21

gold

Question 22

what test gives a positive or negative result

Answer 22

qualitative

Question 23

what test gives a high positive or low positive result

Answer 23

quantitative

Question 24

what control distinguishes false negative results from failed amplifications

Answer 24

internal control

Question 25

adjusting an instrument or test system output to the actual concentration of a reference analyte by testing and making appropriate adjustments

Answer 25

calibration

Question 26

probes, primers, antibodies, and other tests components that detect a specific Target, such as a cell surface protein or DNA mutation

Answer 26

ASR

Question 27

Reagent sets intended for use in the diagnosis of a specific disease or other condition

Answer 27

IVD