Chapter 12 Flashcards
nucleic acid sequence info used to classify bacteria
classification
to further characterize bacterial species
genotyping
controls for amplification
homologous extrinsic
controls for extraction and amplification
heterologous extrinsic
human gene control
heterologous intrinsic
-static
inhibit growth
-cidal
kill organisms
what is now considered the gold standard when phenotype assays are being developed
molecular methods
rapidly spreading outbreak of infectious disease
epidemic
a disease that sweeps across wide geographical areas
pandemic
collection and Analysis of environmental, microbiology, and clinical data
epidemiology
measures biological characteristics of organisms
phenotypic analysis
a genotypic analysis targeting genomic or plasmid DNA
molecular epidemiology
PCR amplification priming outward from repetitive element generate what products
strain specific products
viruses of detection include antibody detection, antigen detection, or culture
classical methods
viruses of detection include Target, probe, and Signal amplification
molecular methods
number of viruses per milliliter of fluid
viral load
lowest level detected at least 95% of the time
sensitivity
ability to determine True Value
accuracy
reproductibiliy of independently determine test results
precision
negative samples are always negative and positive results are true positives
specificity
a Serial dilution of standard curve closely approximates a straight line
linearity
accuracy of measurement of virus regardless of sequence variations
flexibility
what genes mutate to overcome antiviral agents
viral genes
what mutations are detected by sequencing
gene mutations
what mutations affect drug sensitivity but may slow viral growth
primary resistance mutations
what mutations compensate for the primary resistance growth defects
secondary resistance mutations
what is used to type molds
its sequencing. Molds are typed by PCR and sequencing of its regions 428 s rrna
a molecular based typing method that has high typing capacity, reproduct ability and discriminatory power, moderate ease of performance, and good to moderate ease of interpretation
pfge
a positive result for HPV type 16 indicates
high risk for cervical cancer
a patient has antibodies against each CV and a high viral load. What should be performed next
inno LIPA hcv genotyping
What gene would be analyzed to determine whether an isolette of staphylococcus aureus is resistant to oxacillin
MEC a
what is a genotypic method used to compare two isolates and an epidemiological investigation
ribotyping
