Exam 1 Flashcards
1
Q
Mercaptoethanol
A
cleavage of disulfide bridges
2
Q
Dithiothreitol (DTT)
A
cleavage of disulfide bridges
3
Q
Edman’s Reagent
A
N-terminal analysis
4
Q
Phenythiothicyanate
A
N-terminal analysis
5
Q
Carboxypeptidase A
A
cleaves all except P, R and K
6
Q
Carboxypeptidase B
A
Only works on R and K
7
Q
Trypsin
A
cleavage on C-side of R and K
8
Q
Chymotrypsin
A
C-side F, Y, W
9
Q
Clostripain
A
like trypsin but attacks more R than K
10
Q
Staphylococcal protease
A
C-side of E, D in phosphate buffer and specific for E in acetate or carbonate buffer
11
Q
cyanogen bromide (CNBr)
A
Acts only on c-side of M
