Enzymology Tutorial

Question 1

Spectrophotometry Def

Answer 1

Quick method of evaluating analyte concentration

Question 2

Analyte Def

Answer 2

Substance that needs to be measured and tested

Question 3

How Spectrophotometer can be used

Answer 3

Clinical diagnosis, disease screening, medicine accuracy and biochemical analysis (enzyme catalysis)

Question 4

Beer Lambert Law

Answer 4

Absorbance = (wavelength absorbance coefficent)(length)(anylate conc)

Question 5

Relationship between absorbance and concentration

Answer 5

Absorbance is directly proportional to concentration

Question 5

Dilution Equation

Answer 5

(molarity or w/v)1 x (v)1 = (molarity or w/v)2 x (V)2

Question 6

Why do you measure enzyme activity in body fluids

Answer 6

Measure protein directly, enzymes bind specific substrate, monitor substrate changing to product and understand what inflences enzyme activitynitor disese progression

Question 7

What does increase of enzyme activity in serum indicate

Answer 7

Increase tissue damage or poliferation

Question 8

Inhibitor Def.

Answer 8

Substance that slows the velocity of enzyme-catalysed action

Question 9

Irreversible Inhibitor Def

Answer 9

Develops covalent bonds. Can’t revert back. Eg aspirin

Question 10

Reversible Inhibitor Def.

Answer 10

Ionic bonds. Can revert back by disassociation

Question 11

2 Types of reversible Inhibitors

Answer 11

Competitive and non-competitive

Question 12

Competitive inhibitor Def and example

Answer 12

Inhibitor binds to active site blocking substrate. Anti-hyperlipidemic drugs bind to HMG-A reductase to prevent cholesterol synthesis. Km increases and Vmax unaffected

Question 13

Non-competitive Inhibitor Def.

Answer 13

Displaces co-metal factpors. Vmax reduced and Km unaffected