Enzymes Flashcards
Enzymes function
Catalyse almost all biological functions
Increase rate of reaction
Do enzymes change equilibrium
No
What does enzyme catalysis do
Lowers activation energy
Forms ES complex
Enzymes increase reaction rate by
Lowering activation energy
What factors can help increase reaction rate
Substrates in close proximity
In correct orientation
Transition state stabilised
What enzyme is used in RBC
Carbonic anhydrase
Ph affects
Ionisation state of amino acid r groups
Coenzymes function
Help enzymes
NAD is considered a
Coenzyme
Where do coenzymes derive from
Vitamins
Drugs control inappropriate
Enzyme activity
Reversible enzyme inhibitors have a rapid
Dissociation
Irreversible enzyme inhibitors have a very slow
Dissociation
What inhibitors can be toxic
Irreversible
Competitive inhibitors bind to
Active site
Competitive inhibitors bind with what for binding
Substrate
Examples of use of competitive inhibitors
Feedback inhibition
Competition treatment for poison ingestion
Non competitive inhibitor binds to
Allosteric site
You cannot overcome inhibition by adding more substrate in what type of inhibitor
Non competitive
Allosteric enzymes have what type of curve in a graph
Sigmodial
Features of Allosteric enzymes
More than 1 binding site
Changes shape when substrate binds
Co-operative binding
Doesn’t obey MM kinetics
