ENZYMES Flashcards
Higher the enzyme concentration, the faster is the reaction, because more enzyme is present to bind with substrate
Enzyme Concentration
The reaction rate steadily increases as more subtrate is added
Substrate concentration
Organic Compound
Increasing its concentration will increase the velocity of an enzymatic reaction
Essential to achieve absolute enzymatic activity
Ex: NAD & NADH
Coenzymes
Inorganic ions which alters the spatial configuration of enzyme for proper substrate binding
Ex: Calcium, Zinc, Chloride, Magnesium & Potassium
Activators
Inorganic ion attached to a molecule
Ex: Catalase & Cytochrome Oxidase
Metalloenzymes
Physically binds to the active site of an enzyme
Inhibition is reversible when the substrate concentration is significantly higher than concentration of inhibitor
Competitive Inhibitor
Does not compete with substrate but look for areas other than the active sire
Subtrate & Inhibitor may bind an enzyme simultaneously
Non-Competitive Inhibitor
Inhibitor binds to the enzyme-substrate (ES) complex
Increasing substrate concentration results to increase inhibition
Uncompetitive Inhibitor
Enzymes having the same catalytic reactions but slightly different molecular structures because of differences in the amino acid sequence of enzymes
Isoenzymes
Enzymes are active at what temperature?
25C
30C
37C (Optimum)
Most physiologic reactions occur in the pH range of ___
7 to 8
Ideal temperature for preservation of enzymes
-20C
Ideal storage temperature for substrates and coenzymes
2 - 8C
Ideal for storage of LDH
LDH4
LDH5
Cold labile LDH
22C or Room temperature
Catalyze the removal or addition of electrons
Ex:
1. CO
2. LDH
3. MDH
4. ICD
5. G-6-PD
Oxidoreductases
Catalyze the transfer of a chemical group other than hydrogen form one substrate to another
Ex:
1. CK
2. AST
3. ALT
4. OCT
Transferases
Catalyze hydrolysis or splitting of a bond by the addition of water
Ex:
1. ACP, ALP, CHS, LPS
2. Trypsin, Pepsin, LAP
3. AMS, Galactosidases
Hydrolases
Catalaze removal of groups from substrates without hydrolysis
Ex:
1. Glutamate decarboxylase
2. Pyruvate decarboxylase
3. Tryptophan decarboxylase
4. Aldolase
Lyases
Catalyze the intramolecular arrangement of the substrate-compound
Ex:
1. Glucose phosphate isomerase
2. Ribose phosphate isomerase
Isomerases
Catalyze the joining of two substrate molecules, coupled with breaking of pyrophosphate bond in ATP or similar compound
Ex:
1. Synthase
Ligases
Reaction rate depends only on enzyme concentration
Zero-order reaction
Reaction rate is directly proportional to substrate concentration
First-Order reaction
Hig Specificity Enzymes:
Acid Phosphatase
Alanine Aminotransferase
Amylase
Lipase
Principal tissues of High Specificity enzymes:
- ACP - RBC & Phosphate
- ALT - Liver
- Amylase - Pancreas, Salivary gland
- Lipase - Pancreas
Moderate Specificity Enzymes:
- Aspartate Aminotransferase
- Creatinine Kinase
Principal tissues of Moderate Specific Enzymes
- AST - Liver, Heart, Skeletal Muscles
- CK - Brain, Heart, Skeletal Muscles
Low Specificity Enzymes
- Alkaline Phosphate
- Lactate Dehydrogenase
Principal tissues of Low Specific Enzymes:
- ALP - Liver, Bone, Kidney
- LDH - All tissues
Nonspecific enzyme capable of reacting with many different substrates
Liberates inorganic phosphate from an organic phosphate ester with concominant production of an alcohol
Alkaline Phosphate
Major Isoenzymes of ALP
- Liver ALP
- Bone ALP
- Placental ALP
- Intestinal ALP
Reference Value of ALP:
30 - 90 U/L
ALP is higher in individuals of blood group ___ because of differences in intestinal ALP levels
Group B & O
Inc. ALP is the Major liver fraction that is most frequently elevated especially in what disease___
Obstructive Jaundice
For bone disorders, highest elevations of ALP occurs in what disease?
Paget’s Disease
Osteitis deformans
Found in lung, breast, ovarian and gynecological cancers
Bone ALP co-migrator
Most heast stable ALP (65C for 30 mins)
Inhibited by Phenylalanine reagent
Carcinoplacental ALP
Regan ALP
Found in adenocarcinoma of pancreas and bile duct, pleural cancer
Inhibited by L-leucine and phenylalanine
Nagao ALP
Most anodal isoenzyme:
Liver ALP
Bone ALP
Least Anodal ALP
Intestinal ALP
Performed at 56C for 10-15 minutes
Heat Fractionability/ Stability Test
Most heat Stable Isoenzyme:
Placental ALP
Most Heat Labile ALP
Bone ALP
Decreasing order of ALP heat stability
Placental, Intestinal, Liver, Bone
This method uses different concentrations of phenylalanine, synthetic urea and levamisole solutions
Chemical Inhibition Test
L-phenylalanine inhibits what isoenzyme of ALP?
Placental
Intestinal
3M Urea inhibits what isoenzyme of ALP
Bone ALP
Levimasole reagent inhibitis what ALP
Liver ALP
Bone ALP
Reference Method & Most Specific method in determining ALP levels:
Bowers & McComb
(Szasz Modification)
Substrate of the Bowers & McComb method:
p-nitrophenylphosphate