Enzymes Flashcards
Succinate inhibits malonate is an example of?
Competitive inhibition
Effect on Km and Vmax due to competitive inhibition?
Km-increases and Vmax- unchanged.
Drugs of competitive inhibition. 1) Methatroxate 2) Dicoumarol 3) Isonicotinic acid hydrazide 4) Sulfonamides . Describe their structural analog and use?
1) Folic acid- DNA synthesis, Anticancer drug . 2) Vit K - Anticoagulant drug. 3) Pyridoxal - Antituberculous drug. 4) PABA - inhibits folic acid synthesis so bacteria dies bcoz they can synthesise folic acid while humans can’t.
Effect on Km and Vmax due to non-competitive inhibition?
Km- decreases and Vmax- unchanged.
What is Km?
Substrate at which half of Vmax is achieved.
Non-competitive inhibition. 1) Cyanide 2) Flouride 3)Iodoacetate 4) British anti lewisite (BAL). Define respective inhibition?
1) Cytochrome oxidase. 2) Enolase ( by removing Mn and Mg ) so inhibits glycolysis. 3) Inhibits enzyme with -sh group. 4) has -sh group + heavy metal poisons and reduce their effects. So used as an antidote for heavy metal poisoning.
Enzyme with non-proteineous part?
Holoenzyme.
Enzyme without non-proteineous part?
Apoenzyme.
Non-proteineous part with inorganic metal ions is called?
Cofactors.
Non-proteineous part with organic part is called?
Coenzyme.
How many types of coenzymes are there? Which are they?
2; Cosubstrates and Prosthetic group.
Cosubstrates?
Coenzyme that transiently attaches with enzyme. Ex-NAD+
Prosthetic group?
Coenzyme that permanently attaché with enzyme. Ex- FAD.
