enzyme kinetics Flashcards
1
Q
as [S] rises so does ___ proportionately (only at lower S)
A
Vo
2
Q
Vo = Vmax [S] / ?
A
Km + [S]
3
Q
1/2 Vmax =
A
Km
4
Q
[E]t = [E] + [ES]
A
total enzyme concentration
5
Q
[S]t = [S] + [ES]
A
total substrate conc.
6
Q
every enzyme has theoretical __ ___ at which it will not work any faster no matter how much substrate
A
speed limit
7
Q
d[ET]/dt = 0
A
steady state assumption
8
Q
a constant that is related to the affinity of an enzyme for the substrate
A
Km
9
Q
smaller Km the ___ the binding
A
`tighter
10
Q
on hill plot the y intercept is
A
1/Vmax
11
Q
on hill plot the x intercept is
A
-1/Km
12
Q
measures how the “affinity if an enzyme to its substrate
A
Km
13
Q
rate limiting step at saturation
A
Kcat
14
Q
specificity constant
A
Kcat/Km
15
Q
two enzymes can be compared using the
A
specificity constant
