Entamoeba

Question 1

Entamoeba life cycle

Answer 1

Ingest cyst
Turns into trophozoite in gut
Trophozoite causes pathogenesis and also makes cysts
Poop out cysts

Question 2

Trophozoite

Answer 2

Motile
Anaerobic
Feeds on gut bacteria

Question 3

Gal/GalNAc lectin

Answer 3

Binds galactose
Increased binding is no sialic acid cap
Destroys epithelial cells quickly (about an hour)

Question 4

Amoebapore

Answer 4

Cytotoxicity similar to AMPs
Required
Binds lipids

Question 5

Cysteine proteases

Answer 5

Degrade mucin
CP5 necessary for killing and dissemination

Question 6

Proteomics

Answer 6

Differential In-Gel Electrophoresis (DIGE)
Similar to transcriptional microarray but uses proteins
Specific spot = specific protein
Want green E. histolytica ones as candidates (peroxiredoxin?)

Question 7

PAMPs and PRRs

Answer 7

Gal/GalNAc lectin - galactose

Question 8

Host control

Answer 8

Complement in bloodstream does not kill E. histolytica

Question 9

Methodology

Answer 9

Compare virulent and a virulent strains with DIGE
Intestinal epithelia binds assay and killing assay
Bloodstream infection assay (fluorescence tagging)