Emzymes Flashcards
Metabolism
The sum of all chemical reactions in an organisms
What is sunlight capable of being absorbed by
Cellular pigments
Eg of solar energy
Solar energy
Cellular energy
The energy stores in the bonds of bio molecules
Eg cellular energy
Respiration
Enzymes
Biological catalysts made of protein
What are enzymes made in
Living things called biological catalysts
Catalysts
Speed up a reaction without being used up in the reaction
What biomolecule is in an enzyme
Protein
The nature of enzymes are..
Proteins
When are proteins made
When amino acids join together in ribosomes
What is it called when proteins are made when amino acids join together in ribosomes
Protein synthesis
What are most enzymes
Globular proteins
What are globular proteins
Folded into 3D shape
Enzymes that act in proteins are called
Proteases
Enzyme reactions are
Reversible
Catabolic enzyme
Breaks a complex molecule into a smaller one
Eg of catabolic reactions
Respiration and digestion
DNA polymerase
Forms and repairs DNA
DNA ligase
Converts simpler molecules into complex ones
What is an anabolic process in plants
Photosynthesis
Name two examples of anabolic reactions in plants
Protein synthesis and DNA
What is the substance the enzyme acts on
Substrate
Active sight
Part of the enzyme that combines with the substrate
The active sight is fixed to
Fit the substrate
What happens when the substrate enters the active sight
It changes shape slightly
What is it called when the enzyme fits precisely around the substrate
Induced fit model
Inhibitors
Can prevent enzymes from working
What type of enzymes do inhibitors attach to
Nerve transmissions
What dénaturés an enzyme involved in respiration
Cyanide
Eg of an inhibitor
Cyanide
Beneficial inhibitors
Pain killers
Antibiotics
Dentatured enzymes
Lots it’s active shape and can no longer carry out its function
Bioprocessing
Use of enzyme controlled reactions to produce a product
Eg of bioprocessing
Making cheese
Bioreactor
A vessel in which living things/ their enzymes are used to make a product
How do you prevent enzymes freely dissolving
Immobilise them
Immobilised enzymes
Attached to each other it in an inert substance and can be used repeatedly
Eg of how they are immobilised
Enclosed within a gel (sodium alginate)
Advantage of immobilised enzymes
Can be reused
Cheaper
What converts lactose to glucose
Lactase
What converts glucose to fructose
Glucose isomerase
What makes chemical reactions faster
Higher temperatures
What temperature do enzymes denature
40 dégrées
What is the optimum pH for an enzyme
7
Pepsin pH
1 or 2
An enzyme cannot act on
Only one substrate
Phosphorylation
Thé addition of phosphate to a molecule
NADP
Nicotinamide adénine dinucleotide phosphate
Where is catalase found
Celery
What does catalase do
Converts hydrogen peroxide into water and oxygen
What does washing up liquid do in an experiment
Breaks down cell membranes
What does the volume of foam indicate
Activity of an enzyme
How do you vary temperature
water bathS
How do u keep the temperature steady
Use one water bath