E3 FA Oxidation (b-oxidation)

Question 1

what is the location of beta-oxidation?

Answer 1

mitochondrial matrix

Question 2

3 FFAs at the target cell are released from albumin and diffuse into the cell via ___________.

Answer 2

transport proteins

Question 3

T/F. FA binding proteins shuttle FA to mitochondria for activation.

Answer 3

true

Question 4

what is the location of FA activation?

Answer 4

outer mitochondrial membrane

Question 5

what is the enzyme responsible for FA activation?

Answer 5

acyl CoA synthase

Question 6

what are the two products of FA activation via acetyl CoA synthase?

Answer 6

acyl CoA and PPi

Question 7

what enzyme is responsible for breaking down PPi?

Answer 7

pyrophosphatase

Question 8

is the activation of FA energy consuming/requiring or energy producing?

Answer 8

energy consume/requiring

Question 9

once the FA is activated it can enter the matrix via ___________.

Answer 9

carnitine palmitoyltransferase complex (CPT I and II)

Question 10

what is the enzyme associated with CPT/entering the matrix?

Answer 10

acylcarnitine translocase 
(aka carnitine acyltransferase)

Question 11

what will inhibit CPT?

Answer 11

malonyl- CoA

Question 12

T/F. CPT II is the rate limiting steps of b-oxidation.

Answer 12

false; CPT I

Question 13

what is the acronym to help us remember the steps for b-oxidation?

Answer 13

OHOT

Question 14

match the enzyme to the b-oxidation step. 
A. O
B. H
C. O
D. T

1. DH
2. hydratase
3. acyl CoA DH
4. ketothiolase

Answer 14

A. 3
B. 2
C. 1
D. 4

Question 15

what step of b-oxidation will produce FADH2? NADH?

Answer 15

oxidation step 1

oxidation step 2

Question 16

what are the products of FA oxidation? which will go to ETS?

Answer 16

acetyl CoA, NADH, FADH2

NADH, FADH2

Question 17

beta oxidation will continue with a long chain FA until we reach ______(#) acetyl CoA.

Answer 17

2

e.g. palmitate= 16C –> 7 runs of b-ox

Question 18

unsaturated FA will follow OHOT, but need to extra enzymes. name them.

Answer 18

reductase and isomerase

Question 19

between what step of OHOT will reductase and isomerase happen?

Answer 19

oxidation (step 1) and hydration (step 2)

Question 20

in the final thiolysis of beta oxidation, what odd chained FA is produced? how many carbons?

Answer 20

propionyl CoA; 3C

Question 21

T/F. in order for odd chain FAs to enter the CAC, it requires a 4 step process and B6.

Answer 21

false; 3 step process and B12

Question 22

once propionyl CoA goes through the 3 step process with the help of B12, what CAC intermediate does it become?

Answer 22

succinyl CoA

Question 23

what will stimulate FA oxidation?

Answer 23

hormones (epinephrine, norepinephrine, glucagon) and low energy

Question 24

what will inhibit FA oxidation?

Answer 24

hormone (insulin), high- glucose, energy, NADH:NAD+, malonyl CoA, acetyl CoA

Question 25

malonyl CoA will inhibit _______ and ______.

Answer 25

CPT I and b-oxidation