E3 FA Oxidation (b-oxidation) Flashcards
what is the location of beta-oxidation?
mitochondrial matrix
3 FFAs at the target cell are released from albumin and diffuse into the cell via ___________.
transport proteins
T/F. FA binding proteins shuttle FA to mitochondria for activation.
true
what is the location of FA activation?
outer mitochondrial membrane
what is the enzyme responsible for FA activation?
acyl CoA synthase
what are the two products of FA activation via acetyl CoA synthase?
acyl CoA and PPi
what enzyme is responsible for breaking down PPi?
pyrophosphatase
is the activation of FA energy consuming/requiring or energy producing?
energy consume/requiring
once the FA is activated it can enter the matrix via ___________.
carnitine palmitoyltransferase complex (CPT I and II)
what is the enzyme associated with CPT/entering the matrix?
acylcarnitine translocase (aka carnitine acyltransferase)
what will inhibit CPT?
malonyl- CoA
T/F. CPT II is the rate limiting steps of b-oxidation.
false; CPT I
what is the acronym to help us remember the steps for b-oxidation?
OHOT
match the enzyme to the b-oxidation step. A. O B. H C. O D. T
- DH
- hydratase
- acyl CoA DH
- ketothiolase
A. 3
B. 2
C. 1
D. 4
what step of b-oxidation will produce FADH2? NADH?
oxidation step 1
oxidation step 2
what are the products of FA oxidation? which will go to ETS?
acetyl CoA, NADH, FADH2
NADH, FADH2
beta oxidation will continue with a long chain FA until we reach ______(#) acetyl CoA.
2
e.g. palmitate= 16C –> 7 runs of b-ox
unsaturated FA will follow OHOT, but need to extra enzymes. name them.
reductase and isomerase
between what step of OHOT will reductase and isomerase happen?
oxidation (step 1) and hydration (step 2)
in the final thiolysis of beta oxidation, what odd chained FA is produced? how many carbons?
propionyl CoA; 3C
T/F. in order for odd chain FAs to enter the CAC, it requires a 4 step process and B6.
false; 3 step process and B12
once propionyl CoA goes through the 3 step process with the help of B12, what CAC intermediate does it become?
succinyl CoA
what will stimulate FA oxidation?
hormones (epinephrine, norepinephrine, glucagon) and low energy
what will inhibit FA oxidation?
hormone (insulin), high- glucose, energy, NADH:NAD+, malonyl CoA, acetyl CoA
malonyl CoA will inhibit _______ and ______.
CPT I and b-oxidation