Drugs Analysis Tools

Question 1

Analysis Types

Answer 1

Chemical, Physical and Microbiological

Question 2

Analysis Applications

Answer 2

Quality Testing (whether sample = label), stability testing (shelf life/half life), compatibility testing (interaction with body/other medicines), drug ID (qualative), drug characteristics (quantative), bioanalysis (pharmacological effects), drug isolation from samples

Question 3

What manufacturers test for

Answer 3

is it pure, safe, functional

Question 4

Bioanalysis Def

Answer 4

Analysis of a drug in a biological specimen. Eg in blood, in respiration, LADME profiles

Question 5

2 errors in bioanalysis

Answer 5

Sample prep (not correctly isolated) and machine error (eg wrong settings

Question 6

Area under curve in Bioanalysis Graph

Answer 6

Concentration of drug absorbed

Question 7

Bioanalystic Science Def

Answer 7

Study of analysing biological/non samples using biological methods

Question 8

Preformulation Def

Answer 8

Prediction of drug characteristics before formulation

Question 9

Quality Asssurance and Quality Control Outline

Answer 9

Ensure quality, purity, safety and effectiveness of drug products

Question 10

Specifications Def

Answer 10

Lists of tests (eg stability, solubility), references analytical procedures and acceptable criteria (numerical ranges). Establish quality guidelines

Question 11

Tablet tests

Answer 11

Disintegration, Friaing, Hardness and dissolution

Question 12

Qualitive Analysis Def

Answer 12

Looking at substance (eg it’s substituents) to identify it and ascertain nature of constituents

Question 13

Quantitive Analysis Def

Answer 13

Substance analysis of amount and proportions of chemical constituents

Question 14

Accuracy Def

Answer 14

The closeness of a sample value to the true value of population (mean)

Question 15

Precision Def

Answer 15

Closeness of sample value to other values within in the samples

Question 16

Repeatability Def

Answer 16

Can you reproduce your own results

Question 17

Intermediate Precision Def

Answer 17

Can different people in same lab (same equipment) produce same results as you)

Question 18

Reproducibility Def

Answer 18

Can others outside of lab produce same results

Question 19

Titrimetric (titrations) and Chemical Analysis Outline

Answer 19

Anylate is reactated with solution of known conc. Reaction used to infer conc of anylate

Question 20

Spectroscopy Def

Answer 20

Interactions between electromagnetic radiation and particles

Question 21

EMR Spectrem (waekest to strongest)

Answer 21

radio wave, microwaves, Infra red, visible, UV, x-ray, gamma

Question 22

Beer Lambert Law States

Answer 22

Concentration is proportional to absorption

Question 23

IR Spectrometry

Answer 23

EMR causes particles to stretch/bend. Wavelength absorbed infers bond characteristics. Qualitive, shows functional groups of compound

Question 24

Mass Spectroscopy

Answer 24

Analyte is ionised and set through electromagnetic field. Seperated by charge/mass ratio

Question 25

Chromatography

Answer 25

Seperation of individual components in a mix by partitioning of mobile and stationary phase and polarity of solvent. Used in quantative, qualitive and seperation.

Question 26

Factors causing component separation in chromotography

Answer 26

Polarity (like (retained in column) or unlike (passes column)), charge, size, antibodies and solvent selectivity

Question 27

Immuno-assays Outline

Answer 27

Antibody selectivity separates wanated material from mix. Binding strong enough so substance sticks when plate is washed. Attached 2 antibodies. 1 attaches to other side of substrate and another to that antibody. 2nd Antibody has chemical for colour change when attaching to other antibody. used for detection