Doubly Labelled Water Flashcards
1
Q
What are the two isotopes used in doubly labelled water?
A
Deuterium and Oxygen-18
2
Q
What pools does Deuterium label (H2)?
A
The urine pool
3
Q
What pools does Oxygen label (O18)?
A
The urine pool and the CO2 pool (breath)
4
Q
How do we measure isotopes in urine?
A
Isotope will evenly disperse amongst the water, then that label will go out in your urine
- We can then measure the isotopes concentration in urine (isotopic enrichment)
5
Q
How do we measure the amount of O18 in our breath (CO2 pool)?
A
The difference in the amount of H2 and O18 leaving in the urine tells you how much O18 was lost through the CO2 pathway in breath
