DOMAIN EUBACTERIA Flashcards
how many phyla of bacteria
16
bacteria
• Microscopic unicellular prokaryotes.
• Cell structure simpler (no nucleus, and no membrane-bound organelles) – bacteria actually have nucleus (since it is able to replicate), the only thing is it is not bounded by a membrane, it is called a nucleoid.
• Can be classified into shape, arrangement, and gram stain reaction.
shapes of bacteria
• Spherical (cocci)
• Rods (bacilli)
• Comma (vibrio)
• Corkscrew (spirochaetes)
arrangements of bacteria
• Diplococci (pairs)
• Streptococci (chains)
• Staphylococci (clusters)
• Tetrads (sarcina)
• Amount of peptidoglycan in bacterial cell wall determines if the bacteria is gram + or gram -.
• To distinguish gram + and gram - , bacteria uses gram staining.
gram stain reaction
gram reaction can be decolourized to accept counter stain
gram -
gram reaction - retain crystal violet dye and stain dark violet or purple
gram +
peptidoglycan layer is thin (single layer)
gram -
peptidoglycan layer is thick (multi layer)
gram +
teichoic acid is absent
gram -
teichoic acid is present
gram +
periplasmic space is present
gram -
periplamic space is absent
gram +
outer membrane is present
gram -
outer membrane is absent
gram +
lipopolysaccharide content is high
gram -
lipopolysaccharide content is virtually none
gram +
lipid and lipid protein content is high
gram -
lipid and lipid protein content is low
gram +
the cell wall is 70-120 Armstrong thick two layered. the lipid content is high
gram -
the cell wall is 100-120 Armstrong thick, single layered. the lipid content of the cell wall is low.
gram +
• Small group of thermophilic to hyperthermophilic chemolithotrophic bacteria – derive their energy from inorganic molecules
• Live in hot environments
• Genus Aquifex is aerobic while the rest of the phylum are anaerobic and cannot tolerate the presence of oxygen at all.
Phylum 1 - Aquificae
• Can live is high as 95°C
• Optimum growth temp of 85°C
• Thermophilic and hyperthermophilic bacteria that thrive in hydrothermal environments
• Aerobic and can only tolerate low quantities of oxygen
• Gram-negative
• Non-sporulating
• Motile nature
• Metabolic versatility
• Chemolithoautotrophs employing the reductive tricarboxylic acid cycle for CO2 fixation.
• Growing aerobically while utilizing molecular hydrogen, elemental sulfur or thiosulfate as energy substrates.
• Utilizes H2S or S2O32 as its energy source
Genus Aquifex (Phylum Aquificae)
• Comprises of a number of aerobic chemoorganotrophic bacteria
• 2 best studied genera: Thermus and Deinococcus
Phylum 2 - Xenobacteria
• Thermophilic bacterium
• Gram negative
• Either immotile or possess flagella and are motile
• More active at higher temperatures
• More thermostable than most other enzymes
• Optimum growth temperature of 70°C-75 °C (min: 37-45°C, max: 79°C)
• Though some have lower growth temperatures of about 60°C (min: 35-40°C, max: 70°C)
• High temperature Thermus pH ranges 7.5-8.0, though strains grow as low as 5.1 and as high as 9.5
Genus Thermus (Phylum Xenobacteria)
Thermus aquaticus (Phylum Xenobacteria)
• Gram-negative bacterial cell structure with a tripartite cell envelope consisting of a plasma membrane, thin middle layer, and a thicker, irregular outer layer.
• Where the enzyme Taq DNA comes from – major enzyme used in Polymerase Chain Reaction (PCR) techniques or amplifying DNA
• A complete tricaboxylic acid cycle, giving a glyoxylate bypass as well with isocitrate lyase and malate synthase.
Chrysiogenes arsenatis (Phylum 3 - Chrysiogenetes)
• Curved, rod-shaped
• 1.0-2.0 um in length and 0.50-0.75 um in diameter.
• Gram-negative
• Strictly anaerobic
• Motile through a single polar flaggellum.
• Has unique lifestyle and biochemistry
• Chemolithoautotroph – uses chemical means to digest rock for energy and does not require organic matter for its existence
• It eats arsenic
• respires with the most oxidized form of arsenic, arsenate
• acetate as ellectron donor
• these organisms use arsenite, an oxidized form of arsenic, as an electron donor to create energy.
• In environments rich in arsenite - anoxic arsenic-contaminated sediments of Lake Coeur D’Alene in Idaho , or in a gold mine in the Northern Territory of Australia.
Phylum 4 - Thermomicrobium
• This is a small phylum of chemotrophic and autotrophic bacteria.
• The two best studied genera are Thermomicrobium and Chloroflexus
Genus Thermomicrobium (Phylum Thermomicrobium)
• Contains 2 gram negative chemotrophic species
• Lipids form neither ester linkages like other bacteria, nor linkages like the Archaea and Eukarya
• the lipids are made up of 1,2-dialcohols instead of glycerol.
Genus Chloroflexus (Phylum Thermomicrobium)
• thermophilic,
• filamentous,
• Gram negative,
• anoxygenic phototroph
• Grow at temperature 35°C-70°C
• inhabitant of neutral to alkaline hot springs where it often forms thick mats.
• Photosynthesis is carried out via bacteriochlorophyll a.
• Chloroflexus spp. can also grow in the dark by means of chemo-organotrophic by aerobic respiration
• an ancient genus with a photosynthetic metabolism that is part way between that of the Purple Bacteria and the Green Sulphur Bacteria.
• Some scientists think Chloroflexus may be one of the earliest photosynthetic bacteria.