(DNA VIRUSES) Lesson 5: Circoviridae

Question 1

6 General description of Circoviridae

Answer 1

 circular single-stranded DNA
 non-enveloped with icosahedral symmetry
 Size range: 17 to 22 nm in diameter
 stable in the environment at pH 3 to pH 9
 resists heating at 60°C for 30 minutes
 Circoviruses cause disease in chickens,
psittacine birds and pigs

Question 2

shape of Circoviridae

Answer 2

circular

Question 3

DNA strand of Circoviridae

Answer 3

single-stranded DNA

Question 4

Size range of Circoviridae

Answer 4

17 to 22 nm in diameter

Question 5

Circoviridae is stable in the environment at what pH level?

Answer 5

pH 3 to pH 9

Question 6

Circoviridae resists heating at what temperature and time?

Answer 6

60°C for 30 minutes

Question 7

Circoviruses cause disease in what animals?

Answer 7

chickens, psittacine birds and pigs

Question 8

host-specific and infect cells of the haemolymphatic system

Answer 8

Circoviridae

Question 9

Circoviridae infect cells of what system?

Answer 9

haemolymphatic system

Question 10

Circoviridae causes what diseases?

Answer 10

Chicken anemia virus (CAV)
Porcine circovirus 2 (PCV 2)

Question 11

affects young birds worldwide, resulting to
aplastic anaemia and generalized lymphoid atrophy

Answer 11

Chicken anemia virus (CAV)

Question 12

Chicken anemia virus (CAV) generally affects what?

Answer 12

young birds

Question 13

Signs of Chicken anemia virus (CAV) affected birds

Answer 13

(DAP)
depressed
anorexic
pale affecting weight gains

Question 14

Chicken anemia virus (CAV) can be transmitted through what?

Answer 14

horizontal (oro-faecal route) and
vertical (thru the eggs)

Question 15

oro-faecal route

Answer 15

horizontal transmission

Question 16

thru the eggs

Answer 16

vertical transmission

Question 17

Infected poultry sheds Chicken anemia virus (CAV) for_________ following infection

Answer 17

3-4 weeks

Question 18

Diagnosis of Chicken anemia virus (CAV)

Answer 18

Clinical signs
Gross lesions at post-mortem
Confirmatory diagnosis: serology (ELISA, virus neutralization test [VNT] )
Molecular diagnosis: PCR

Question 19

Confirmatory diagnosis of Chicken anemia virus (CAV)

Answer 19

Serology:
ELISA and Virus Neutralization Test (VNT)

Question 20

Molecular diagnosis of Chicken anemia virus (CAV)

Answer 20

PCR

Question 21

Control of Chicken anemia virus (CAV)

Answer 21

biosecurity and vaccine

Question 22

associated with postweaning multisystemic wasting syndrome (PMWS), porcine dermatitis, and nephropathy syndrome (PDNS), and also to reproductive problems

Answer 22

Porcine circovirus 2 (PCV 2)

Question 23

What syndromes and disease are associated with porcine circovirus 2 (PCV 2)?

Answer 23

Postweaning Multisystemic Wasting Syndrome (PMWS)
Porcine Dermatitis and Nephropathy Syndrome (PDNS)
Reproductive problems

Question 24

Three genotypes of Porcine circovirus 2 (PCV 2)

Answer 24

PCV-2a
PCV-2b
PCV-2c

Question 25

Diagnosis of Porcine circovirus 2 (PCV 2)

Answer 25

Serology (ELISA, indirect immunofluorescence)

Question 26

This virus /disease is characterized with histopathological changes in lymphoid tissues (granulomatous inflammation, depletion of lymphocytes, and intracytoplasmic inclusions in macrophages)

Answer 26

Porcinecircovirus 2 (PCV 2)

Question 27

Control of PCV 2

Answer 27

Biosecurity and vaccine
Passive immunity from the vaccine from sows to piglets thru colostrum.

Question 28

Passive immunity from the vaccine can be conferred from sows to piglets thru what?

Answer 28

colostrum

Question 29

Chicken anemia virus or CAV, affects young birds worldwide, resulting to
these conditions.

Answer 29

Aplastic Anaemia
Generalized Lymphoid Atrophy