DNA technology Flashcards
3 steps in pcr?
denaturation, annealing and ploymerisation
6 players in pcr?
template dna primers thermostable dna ploymerase taq magnedsium dNTPs thermocycler
what is sanger sequencing?
read length and colour by fluorecing with radioactive terminator bases
what is next gen sequencing?
measure colour and light intesity
what is third gen sequencing?
nanotechnology based techniques
how is dna cloning more accurate than pcr?
more accurate, stable , make more copies , synthetic bio
3 basic tools of dna cloning?
vectors, restriction enzymes and host cells
what are palindromes?
dna same when read from both directions
what is transformation?
putting vector into host
why measure mRNA?
easier than protein due to complementary base pairing
what is in-situ hybridisation?
labelled nucleic acid probs complementary to target mrna, to measre fluorescent signal
what are two high-throughput methods?
gene microarrays and rna sequencing
