DNA synthesis Flashcards
What are replication origins in DNA recognised by? [1 mark]
Initiation complex
How many replication forks are present per replication bubbles in DNA? [1 mark]
2
What is the function of eukaryotic DNA polymerase A, δ and E? [1 mark]
Replication
What is the function of eukaryotic DNA polymerase B? [1 mark]
Repairs (abasic ends)
Where is eukaryotic DNA polymerase γ located? [1 mark]
Mitochondria
What is bacterial DNA polymerase I and II used for? [1 mark]
Repairing
What is bacterial DNA polymerase III used for? [1 mark]
Replication
What does DNA polymerase need? [4 marks]
- DNA template
- Primer
- dNTP (e.g. ATP)
- Mg2+
What does DNA helicase do? [1 mark]
Uses ATP to force two strands apart
What do DNA binding proteins do? [1 mark]
Protects parent DNA from being attacked
What does primase/replicative DNA polymerase do? [1 mark]
Synthesises DNA/RNA primers
What does DNA ligase do? [1 mark]
Joins lagging strands together
What does topoisomerase do? [1 mark]
Removes positive super coils ahead of DNA replication forks
