DNA Replication test Flashcards

1
Q

Where is DNA found

A

In nucleus

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2
Q

When does the replication occur?

A

When the cell is ready to split

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3
Q

Bands are apart of what structure and are?

A

DNA structure and are complementary (always the same)

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4
Q

Each strand in the structure is…?

A

An inverse replica of the other (template)

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5
Q

What are the six proteins of the DNA replication

A

Helicase, topoisomerase, binding proteins, primase, DNA polymerase, DNA ligase

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6
Q

What are the five terms?

A
  1. Parent strand
  2. Daughter strand
  3. Leading strand
  4. Lagging strand
  5. Replication fork
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7
Q

What does the helicase do?

A

Unwinds and unzips the double helix

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8
Q

What does the topoisomerase do?

A

Relieves supercool by cutting and reconnecting the sugar phosphate backbone

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9
Q

What does the binding proteins do?

A

Single strand binding proteins keep helices from binding

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10
Q

What does the primase do?

A

Short strand of RNA that serves as a starting point for DNA synthesis.
Must go from 5’ to 3’

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11
Q

What does the DNA polymerase do?

A

Enzyme that connects free nuecleotides to the parent strand forming a complementary strand

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12
Q

What is the adj for leading strand?

A

Continuous

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13
Q

Lagging strand

A

Fragmented

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14
Q

What does the DNA ligase do?

A

Joins single chain links between Okazaki fragments. Completes short patch DNA synthesis occurring in DNA repair process

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15
Q

What are the 4 parts of the membrane function?

A
Diffusion
1. Osmosis
Permeability
Transport
1. Passive transport
  A. Facilitated diffusion
2. Active transport 
Movement
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16
Q

Def of diffusion ?

A

The process by which molecules of a substance move from areas of higher concentration of that substance to areas of lower concentration

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17
Q

Def of permeable?

A

The membrane that allows substances to pass through

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18
Q

Def of Impermeable?

A

A membrane that does not allow substances pass through

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19
Q

Def of selectively permeable?

A

Some substances can pass through a membrane while Other substances are blocked

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20
Q

What is osmosis

A

The diffusion of water through a selectively permeable membrane

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21
Q

What is passive transport

A

The movement of substances through the phospholipid bilayer portion of the membrane without energy

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22
Q

What are the two kinds of transports

A

Passive and active

23
Q

What is active transport

A

Requires energy, atp becomes adp, goes against gradient

24
Q

Passive transport nonpolar or polar

25
What is facilitated diffusion
Passive transport through protein channels embedded in the cell membrane
26
What does the active transport requires
Energy/ATP and it becomes ADP
27
Exocytosis
Vacuole joins with the plasma membrane and cytoplasm to surround and engulf a large particle(cell eating)
28
Endocytosis
Plasma membrane forms a pocket around substance, engulfs it and then forms a vacuole in the cytoplasma
29
Beta oxidation
Mitochondrial matrix
30
What protein unwinds and unzips
Helicase
31
What protein relieves the supercoil
Topoisomerase
32
What protein keeps the daughter strand from closing
Binding proteins
33
What protein creates a handhold for the 5’ and makes a starting point
Primase
34
What is density
More dense less permeable
34
Able to state the two different molecules that make up an ATP
Adenosine and phosphate
35
Be able to define phosphorylation
ADP and inorganic phosphate — ATP: adding one (inorganic) phosphate to make ATP
36
State and explain the main purpose of glycolysis
Glucose—pyruvate—NADH
37
State and explain the main purpose of the beta oxidation
Cuts fatty acid every 2 carbons, create Acetal CoA
38
State and explain the main purpose of the Krebs cycle
Take carbon off (6 MADH, 2 FADHv2, ATP—24 NADH, 8FADHv2, (lipid) ATP)
39
Staying explain the main purpose of oxidative Phosphorylation
Redox reactions that transfer electrons from an electron donor to an electronic acceptor
40
What are the two parts under oxidative phosphorylase
Electron transport chain and Chemiosmosis
41
What does electron transport chain do?
Pump hydrogen out to create gradient
42
What is chemiosmosis
ATP synthase, create ATP w/ out using ATP
43
What are the four different ways that energy is stored
1. ATP, ADP 2. NADH, FADHv2, NADPH 3. Organic molecules 4. Kno the summary chemical equations for cellular respiration
44
How is energy stored in ATP and ADP
ATP: high energy bonds ADP: one less
45
How is energy stores with NADH, FADHv2, NADPH
Stores in electron: that will be donated
46
How is energy stored with organic molecules
Potential energy in carbon chains
47
Kno the summary chemical equations for cellular respiration
C6H12O6+O2—CO2+H2O
48
Kno why oxygen is important in cellular respiration and what happens if oxygen is not present
Can’t form CO2 Krebs cycle doesn’t work, can’t form H2O oxidation doesn’t work
49
State and explain how rate limiting agent in glycolysis and beta oxidation
Explain: enzyme breaks off Agent for BO: carnatine shuttle Agent for G: PFK(phosphofutose kinase
50
What is the location for glycolysis
Cytosol
51
What is the location of beta oxidation
Mitochondria
52
What is the location of Kreb’s cycle
Mitochondria matrix
53
What is the location for electron transport
Cristae