Diversity of Cells Flashcards
What must tissue be impregnated with and why?
A support material such as wax to maintain structure of cells in thin slice
Unnecessary for cell smears
Why must tissue be sliced thinly to view under microscope?
To allow light to penetrate tissue
How is tissue treated with wax?
First dehydrated, put in organic solvent, and placed in hot wax for several hours
How are thin tissue sections cut?
Using a microtome
Explain process to prepare tissue for viewing
Thin sections put on slides, wax washed away and tissue is rehydrated
What are artifacts?
Small changes in the tissue caused by the preparation process
What is haematoxylin?
A basic dye which binds to acidic molecules and stains them purple
eg. the nucleus
What is eosin?
An acidic dye which binds to basic molecules and stains them pink
eg. proteins in cytoplasm
Why are epithelial cells suited to lining body cavities?
Form sheets of cells due to binding of cells together with little space between them
What is the basal lamina?
Layer of extracellular matrix components to which all of the epithelial cells are attached
Are epithelial cells non-vascular?
Yes
Oxygen and nutrients must diffuse across basal lamina
Are epithelial cells polarised?
Yes
The apical and basal ends are different
Give an example of an organ made of solid epithelial cells
the liver
List 7 different functions of epithelial tissues
Mechanical barrier (skin)
Chemical barrier (stomach lining)
Absorption (intestine lining)
Secretion (salivary gland)
Containment (urinary bladder lining)
Locomotion by movement of cilia (oviduct)
Minor localised functions (sensation eg neuroepithelium which makes up taste buds and contractility eg myoepithelial cells)
List the three shapes of epithelial cells
Squamous (flat)
Cuboidal (cube)
Columnar (column)
What does a simple layer of epithelial cells mean?
Layer only one cell thick
What does it mean if an epithelial tissue is stratified?
Layer is two or more cells thick