Diagnosis Of Infectious Disease Flashcards

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1
Q

What are the 7 types of cultures?

A
  • blood cultures
  • throat cultures
  • sputum cultures
  • cerebrospinal fluid cultures (CFS)
  • stool cultures
  • urine cultures
  • genital tract cultures
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2
Q

When are blood cultures most often performed?

A

When sepsis, endocarditis, osteomyelitis, meningitis or pneumonia are suspected

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3
Q

When are throat cultures primarily used?

A

To detect the presence of:

  • group A beta-haemolytic streptococcui (Streptococcus pyogenes)
  • diphtheria
  • gonococcal pharyngitis
  • thrush
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4
Q

What usually causes an acute sore throat?

A

Viruses - 90% of the time

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5
Q

When are sputum cultures performed?

A

When pneumonia, tuberculosis or lower respiratory infections are suspected

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6
Q

When are cerebrospinal fluid cultures performed?

A

When meningitis, primarily meningococcal meningitis, or other central nervous system diseases are suspected

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7
Q

When are stool cultures performed?

A

Primarily for diarrhoeal disease
If doing a culture for parasites may need to collect into a special container with PVA (polyvinyl alcohol) fluid to preserve the parasite

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8
Q

When are urine cultures performed?

A

Collected for symptoms of urinary tract infections or pyelonephritis. Culture can be tested immediately on a dipstick.

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9
Q

When is a genital tract culture performed?

A

When Neisseria gonorrhoea, Chlamydia trachomatis, Trichomonas vaginalis or Candida vaginitis is suspected.
- can be taken via speculum with a swab or by a self taken vaginal swab

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10
Q

What is an obligate anaerobe?

A

An organism that is able to, or can only, live in the absence of oxygen

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11
Q

What is an obligate aerobe?

A

An organism that grows in the presence of oxygen or requires oxygen for growth

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12
Q

What is an aerotolerant organism?

A

They do not require oxygen to grow however they can tolerate its presence and are not poisoned by oxygen

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13
Q

What is a facultative anaerobe?

A

Can grow with or without the presence of oxygen because they metabolise energy aerobically and anaerobically

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14
Q

What is a microaerophile?

A

Need oxygen because they cannot ferment or respire anaerobically however they are poisoned by high concentrations of oxygen.

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15
Q

What are some characteristics of anaerobes?

A
  • smell bad

- cause some of the worse infections

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16
Q

Name 4 types of selective media?

A
  • sheep’s blood agar
  • chocolate agar (cooked blood)
  • Eosin methylene blue agar, bacteria change colour on agar so no need for a microscope (E.coli is yellow and P.aeruginosa is green)
  • Mannitol salt agar
17
Q

What are ways to identify bacteria? (7 points)

A
  • growth on media
  • morphology and staining
  • oxygen requirements
  • nutritional requirements
  • biochemical activities
  • antibiogram
  • pathogenicity
  • genetics
18
Q

What does immunoassay mean?

A

A biochemical test used to detect the presence or quantity of a specific substance by binding antibodies to antigens

19
Q

Name 3 non-culture diagnostic methods?

A
  • immunologic tests
  • sandwich Elisa (EIA)
  • fluorescent antibody test
20
Q

What does PCR stand for?

A

Polymerase chain reaction

21
Q

What does PCR do?

A

Used to amplify a segment of DNA (multiply it exponentially)

22
Q

What are the different ways of testing? (4 points)

A
  • culture
  • microscopy
  • immunoassay
  • molecular methods (PCR)