Detection and Identification of Antibodies

Question 1

Removal of antibodies from SERUM

Answer 1

Adsorption

Question 2

• Removal antibodies from RBC
• Breaking of bonds between Ag-Ab

Answer 2

Elution

Question 3

Enzymes used to remove Antibodies

Answer 3

1. Papain
2. Bromelain
3. Ficin
4. Trypsin

Question 4

Sources of enzymes used to remove Antibodies

1. Papain

Answer 4

Papaya

Question 5

Sources of enzymes used to remove Antibodies

2. Bromelain

Answer 5

Pineapple

Question 6

Sources of enzymes used to remove Antibodies

3. Ficin

Answer 6

Fig Plant

Question 7

Sources of enzymes used to remove Antibodies

4. Trypsin

Answer 7

Pig’s stomach

Question 8

Blood Group that are enhanced with Enzymes

Answer 8

“ABo! RicH Kidd si Lewis may P1.I00”

ABO
Rh
Kidd
Lewis
P1
I

Question 9

Blood Group that are destroyed in the presence of Enzymes

Answer 9

MNSs
Duffy
Xg
Chido/Rogers
Y^t

Question 10

Combination of Dithiothreitol (DTT) and Cysteine-activated papain

Answer 10

ZZAP (Cystein-Activated Papain)

Question 11

• Removal of autoantibodies from patient cells
• used to destroy Kell, Gerbich, Dombrock, Cartwright

Answer 11

ZZAP

Question 12

NEUTRALIZATION TECHNIQUE:

hydatid cyst fluid
Droppings of Pigeon
Egg white turtledove

Answer 12

Anti-P1

Question 13

NEUTRALIZATION TECHNIQUE:

Saliva, Plasma, Serum

Answer 13

Anti-Lewis

Question 14

NEUTRALIZATION TECHNIQUE:

guinea pigs urine

Answer 14

Anti-Sd^a

Question 15

NEUTRALIZATION TECHNIQUE:

Plasma / Serum

Answer 15

Anti-Chido, Anti-Rogers

Question 16

NEUTRALIZATION TECHNIQUE:

Mother’s milk

Answer 16

Anti-I