Crossmatching

Question 1

Part of Compatibility Testing

1. Final checking of ABO compatibility
2. detect presence of antibody in PX serum that reacts against Donor’s Red cell

Answer 1

Crossmatching

Question 2

Major Crossmatch Formulation

Answer 2

PSDR

Patient’s Serum
Donor’s Red Cell

Question 3

Minor Crossmatch Formulation

Answer 3

DPPR

Donor’s Plasma
Patient’s Red Cells

Question 4

Major and Minor COMPATIBLE

Answer 4

Release Blood

Question 5

Major and Minor INCOMPATIBLE

Answer 5

Do not Release Blood

Question 6

Major Incompatible
Minor Compatible

Answer 6

DO NOT RELEASE BLOOD

Question 7

Major Compatible
Minor Incompatible

Answer 7

Release PACKED RBC (removed plasma)

Question 8

PRACTICE QUESTION:

Patient: Type A
Donor: Type AB

Major:
Minor:

Question 9

PRACTICE QUESTION:

Patient: Type AB
Donor: Type A

Major:
Minor:

Question 10

PRACTICE QUESTION:

Patient: Type B
Donor: Type O

Major:
Minor:

Question 11

PRACTICE QUESTION:

Patient: Type O
Donor: Type AB

Major:
Minor:

Question 12

PRACTICE QUESTION:

Patient: Type O
Donor: Type B

Major:
Minor:

Question 13

PRACTICE QUESTION:

Patient: Type A
Donor: Type AB

Major:
Minor:

Question 14

1. select uncrossmatched, Group O Rh (-) Packed RBC
2. may give uncrossmatched Group O Rh (+) Packed RBC; male patient/Female patient BEYOND CHILD BEARING AGE
3. may be able to provide TYPE-SPECIFIC, UNCROSSMATCHED RBC

Answer 14

In cases of EMERGENCIES

Question 15

1. Also referred as ABBREVIATED CROSSMATCH
2. Simplest and frequently used method to demonstrate ABO compatibility
3. PATIENT’S SERUM TO BE MIXED WITH SALINE-SUSPENDED RED CELL AT ROOM TEMP

Answer 15

Immediate Spin Crossmatch

Question 16

1. Clinically significant antibody has been detected
2. Donor-unit must have antigen-negative towards corresponding alloantibody identified
3. INCUBATED 37 Deg celsius

Answer 16

Antiglobulin Crossmatch

Question 17

1. Also known as Computer Crossmatch
2. Decreases required spx amount and time processing

Answer 17

Electronic Crossmatch

Question 18

Disadvantage of Electronic crossmatch

Answer 18

Requires Recipient’s ABO grouping profile; including current specimen collection

Question 19

Cause of Positive Results in the Serologic Crossmatch.

Answer 19

1. Incorrect ABO grouping of the patient or donor.
2. alloantibody of patient’s serum against Donor’s RBC
3. Autoantibody of patient’s serum against Donor’s RBC
4. Prior coating donor RBC with protein = (+) AHG Test
5. Abnormalities in patient’s serum (Inc. Fibrinogen, Globulin. Plasma Expanders)
6. CONTAMINANTS in the test system

Question 20

Both donor and recipient sample must be stored for a

Answer 20

MINIMUM OF 7 DAYS FOLLOWING TRANSFUSION

Question 21

Donor’s sample and Patient’s sample stored for 7 days must be stored @ what temp

Answer 21

1 to 6 deg celsius

Question 22

Formula for Number of units to screen

Answer 22

Number of Units Requested / Frequency of Antigen Negative Individual