culture transfer techniques Flashcards
Microorganisms are transferred from one medium to another by
subculturing
whats a stock culture
a culture microbiologist keep in fridge use to make many subcultures for students to use
If in lab would be given a subculture of
Serratia marcescens
Serratia marcescens, gram status, shape
gram negative, bacilli
color of Serratia marcescens
pink on grout
is Serratia marcescens harmlful
While often harmless, Serratia marcescens can be an opportunistic pathogen, particularly in immunocompromised individuals
loops are commonly used to obtain a sample from a. broth b. solid
either for solid but only loop for broth
t/f a straight needle (instead of loop) is used when transferring micro organisms to an agar deep tube from both solid and liquid cultures
true
how to do slant to broth transfer:
obtain inoculum from the slant and lightly shake the loop or needle in the broth culture to dislodge teh microorganism
how to do a broth to slant transfer:
obtain a loop full of broth and place at the bas of an agar slant medium. lightly draw the loop over the hardened surface in a straight or zip zag line
slant to agar deep tube transfer
obtain the inoculum from the agar slant. insert a straight needle to the bottom of the tube in a straight line and rapidly withdraaw along teh line of insertion. Called stab inoculation
insert a straight needle to the bottom of the tube in a straight line and rapidly withdraw along the line of insertion. this is called: _______
stab inoculation
what does asepsis mean
no contamination
pure culture
contains only one bacterial species
sterilization vs aseptic techniue
sterilization is only part of aseptic technique. Part of bigger technique, doesn’t stay sterile for long.
