CMV and EBV

Question 1

What are owl eye inclusions?

Answer 1

Large inclusions in the nucleus characteristic of CMV

Question 2

How does the genomic DNA compare to that of other herpesviruses?

Answer 2

It is larger

Question 3

What is unique about the CMV nucleic acid content?

Answer 3

Has viral mRNA in addition to double stranded DNA

Question 4

In a CMV infection, how long might it be before cytopathic effects take place?

Answer 4

4-6 weeks

Question 5

Where are latent CMV infections established?

Answer 5

Monocytes and certain bone marrow cells

Reactivated by immune suppression

Question 6

How are CMV progeny transmitted in the body?

Answer 6

Infected lymphocytes and leukocytes

Also infects vascular endothelial cells

Question 7

How can/what types of people can be infected by CMV?

Answer 7

Congenital infection

Perinatal infection

Normal children and adults

Transplant/Transfusion recipients

Immunocompromised individuals

Question 8

What type of infection is cytomegalic inclusion disease and what is targeted?

Answer 8

Congential infection - asymptomatic mother infects fetus in utero

Visceral organs are targeted

Question 9

What are the possible outcomes of a congenital infection?

Answer 9

Death -rare

Liver/spleen infection

Microcephaly

Thrombocytopenic purpura

Chorioretinitis

Low birth weight

Rash

Question 10

What is the most common virus caused congenital infection?

Answer 10

CMV

More common than rubella b/c of rubella vaccine

Question 11

How is CMV infection acquired in newborns?

Answer 11

Last trimester, CMV secreted in the cervix as a result of reactivation of latent infection

Newborn can also acquire infection from mother’s milk

Question 12

How do normal children and adults acquire CMV infections?

Answer 12

Saliva, urine, tears, blood can transmit virus

Sexual transmisstion

Question 13

What type of mononucleosis does CMV cause?

Answer 13

Heterophile antibody negative mononucleosis

Question 14

What risk is associated with CMV and transfusion or transplantation

Answer 14

Post-transfusion mononucleosis about 3-4 weeks after tanfusion with infected lymphocytes

CMV significant cause of failure of kidney transplants

Question 15

What can result in an unusual disease manifestation of CMV and what are the possible manifestations?

Answer 15

Reactivation of latent disease as a result of pregnancy, immunosuppression

Mononucleosis, pneumonitis, CMV retinitis, GI ulceration, colitis and esophagitis

Question 16

What are the initial stages of infection of CMV?

Answer 16

URI with regional lymph notdes

Early stages are silent ususally

Question 17

How does CMV spread from the initial URI infection?

Answer 17

Infected lymphocytes and monocytes spread the infection to secondary sites like the spleen/lymph nodes, salivary glands, kidney tubuels, cervix, testes/epididymis

Question 18

What occurs to T cells during CMV infection?

Answer 18

There is a temporary reduction in responsiveness

Question 19

What agents can cause congenital infections and defects (TORCH)?

Answer 19

Toxoplasmosis

Other - syphilis, listeria

Rubella

Cytomegalic inclusion disease

HSV