chromatography Flashcards
what are the 4 types of of chromatography
paper
column
thin layer
gas
what is chromatography
a family of separation techniques
which depends on the principle a mixture can be separated if dissolved in a solvent or carried by a gas
and the resulting solution moves over a solid
what is the mobile phase
where the molecules can move this is always a liquid or gas
what is the stationary phase
where the molecules can’t move
this must be on a solid or a liquid on solid support
how are substances separated in chromatography
substances are separated according to reactive affinity to stationary and mobile phase
what is the stationary and mobile phase in paper chromatography
stationary phase absorbent paper
mobile phase solvent
what is the stationary and mobile phase in column chromatography
stationary phase powder or a resin
mobile phase solvent
what is the stationary phase and mobile phase in thin layer chromatography
stationary phase powder(SiO2 or Al2O3)
mobile phase solvent
what is the stationary phase and mobile phase in gas chromatography
stationary phase- powder coated with oil packed into a long capillary tube
mobile phase- unreactive gas nitrogen or helium