Chp12 DNA technology

Question 1

What are Restriction Enzymes?

Answer 1

They cut DNA into fragments and recognize DNA sequence called restriction site.

Question 2

Gel Electrophoresis

Answer 2

Used to separate DNA fragments of different lengths based on their electrical charge and size

Question 3

PCR

Answer 3

Polymerase chain reaction, is a technique used to make copies of specific segment of DNA.

Question 4

GMO

Answer 4

Organicism that have acquired one or more genes by artificial means.

Question 5

DNA Profiling

Answer 5

Analysis of DNA samples to determine whether they come from the same individual

Question 6

Genome

Answer 6

All the genes in a single organism

Question 7

Which of the following is the best definition for recombinant DNA?

Answer 7

DNA that includes pieces from two different sources

Question 8

The process of making multiple copies of a gene by inserting it into a host genome and culturing the host is an example of ______.

Answer 8

gene cloning

Question 9

Gel electrophoresis separates DNA fragments on the basis of differences in their

Answer 9

length

Question 10

Of these steps, which one occurs earliest in the process of producing recombinant DNA?

Answer 10

The same restriction enzyme is used to isolate the gene of interest and to cut the plasmid DNA.