Childress biotechnology QS

Question 1

compare the equipment and techniques of creating cuttings with those used for micropropagation

Answer 1

-micropropagation needs more aseptic discipline.
-micropropagation produces more clone offspring
-micropropagation needs more expensive equipment
-micropropagation needs more expensive skills/ staff training

Question 2

when testing the effect of something on an organism, why is it sometimes important to use clones

Answer 2

-clones have no genetic variation (same genes/alleles)
-you are able to control more variables
-increases validity

Question 3

why is asepsis important

Answer 3

-reduces microorganisms/ contamination
-sterile conditions ensure growth isnt interfered

Question 4

plants were placed in adjacent fields when being tested to control variables such as temp, wind speed and rainfall, what are two other abiotic variables that need to be controlled

Answer 4

-light intensity
-humidity
-soil mineral content
-soil water content
-soil type
-soil pH
-air pollution

Question 5

what does invalid mean

Answer 5

experiment not done correctly

Question 6

what measures should someone take to keep agar plate cultures sterile

Answer 6

-flame inoculating loop/ use sterile pipette tip
-work in an inoculating cabinet/ maintain minimum opening time
-seal plates for inoculation