Chemical and cellular basis of life Flashcards
Hierarchial organisation of living things
Atoms, molecules, organells, cells, tissues, organs, organ-systems, organism, population, community, ecosystem, biosphere
No. of naturally occurring elements on the earth’s crust and elements which are essential to continue healthy life and reproduction
92 elements naturally occur in earth’s crust. Of which, about 20-25% elements are essential to continue healthy life and reproduction. (about 25- elements are essential for humans and about 17 for plants).
C, H, O, N- accounts for of the body mass
96.3%
Present in small amounts but not trace elements
Ca, P, K, S, Na, Cl,
Mg
Major properties of water
Cohesive behavior
Ability to moderate temperature
Expansion upon freezing
Versatility as a solvent
Most abundant group of organic compound on earth is
carbohydrates
Linear forms of polysaccharides
Cellulose , amylose
Branched forms of polysaccharides
Glycogen, amylopectin, hemicellulose
Monomer Inuline
Fructose
Monomer Pectin
Galacturonic acid
Monomer Hemicellulose
Pentoses
Monomer Chitin
Glucosamine
Proteins elemental composition is
C, H,O,N and S
2 types of secondary structure of protein
alpha helix
beta pleated sheet
examples tertiary structure
Most of the enzymes
Myoglobin
Albumin
examples Quaternary structure
Haemoglobin
Collagen
Immunoglobulins
Denaturation of proteins
Denaturation of protein is the loss of specific chemical three dimensional shape due to the alteration of weak chemical bonds and interactions.
Agents affecting the denaturation
- High temperature and high energy radiation
- Strong acids, alkaline and high concentrations of salts
- Heavy metals
- Organic solvents and detergents
Catalytic protein
Pepsin, Amylase
Structural protein
Keratin
Collagen
Storage proteins
Ovalbumin
Casein
Transport proteins
Haemoglobin
Serum albumin
Hormones- proteins
Insulin
Glucagon
Contractile/ Motor
Actin/Myosin
Defensive proteins
Immunoglobins
Functions of DNA
• Store and transmit genetic information from one generation to the next generation
• Store the genetic information for protein synthesis
Least abundant type of RNA
Messenger RNA
Functions of mRNA
• Copies the genetic information stored in DNA molecule as a sequence of nitrogenous bases
• Transports genetic information from nucleoplasam to the site of protein synthesis (ribosome) through nucleopores
Smallest RNA molecule
Transfer RNA
Functions of tRNA
transportation of amino acids to the site of protein synthesis
most abundant type of RNA
Ribosomal RNA
Functions of rRNA
It provides the site where polypeptide chains are assembled.
maximum magnification of light microscope is
1000 times the actual size of the specimen
resolution power of light microscope is
0.2µm
electron microscope should be able to
magnify objects up to
1×108 times. In practice, it magnifies just over 5×105
times.
Diameter of flagella of prokaryotes
20 nm
Diameter of flagella of eukaryotes
200 nm
9+2 arrangement of microtubules.
Thickness of the plasma membrane
7 nm
Difference between animal and plant cell membranes
Plant cell membrane lacks cholesterol
Cholesterol prevents membrane solidification at low temperatures
Functions of plasma membrane
Surrounds the cytoplasm physically separating the intercellular structures from the extracellular environment
Regulates the exchange of materials needed for survival
Involves in cell recognition allowing adjacent cells to communicate
Acts as receptor molecules for biochemicals such as hormones, neurotransmitters and immune proteins
Some proteins acts as enzymes while some attach to cytoskeletal fibres
Diameter of the nucleus
5 µm
Endomembrane system includes
nuclear envelope, the endoplasmic reticulum, the Golgi ap- paratus, lysosomes, various kinds of vesicles and vacuoles, and the plasma membrane.
Rough ER functions
• Transport protein synthesized by ribosomes
• Synthesizing glycoproteins
• Produce transport vesicles
• Facilitate the growth of own membrane by adding phospholipids proteins and carbohydrates. Therefore called as membrane factory
Smooth ER functions
• It synthesizes lipids including oils, steroids and phospholipids.
• Metabolism of carbohydrates.
• Produce transport vesicles to transport within cell.
• Involves in detoxification.
• Stores Ca2+ ions
Golgi apparatus functions
• Collecting, packaging and distribution of materials
• Manufacturing cellulose and non cellulose cell wall components such as pectin
• Produce lysosomes
Lysosomes functions
• Digest food particles received by phagocytosis
• Transport residue material out of cell by exocytosis.
• Digest worn out organelles
• Autolysis causing cell death
Peroxisome functions
• Detoxification of peroxides
• Photorespiration in plants
Glyoxysomes
Specialized type of peroxisome found in fat storing plant cells and converts fatty acids into sugars.
Mitochondria functions
• Synthesize ATP in aerobic respiration
• Involve in Photorespiration
Components of the cytoskeleton
Microtubules (tubulin polymers)
Microfilaments (actin filaments)
Intermediate filaments
Microtubule wall consist of how many columns of tubulin molecules
13
The basal body of cillia and flagella has which structure
9+0
Functions of cillia and flagella
• Act as locomotor appendages
• Can move fluid over the surface of the tissue
• Cilia lining in oviducts help move an egg toward the uterus
Non membrane bounded organelles
Ribosomes
Centrioles
Functions of centrioles
• Produce aster and spindle in cell division
Centrioles are absent in plant cells
Composition of sap of vacuole
water, ions such as Potassium and Chloride and sometimes water soluble colored pigments such as anthocyanin
Composition of stroma of chloroplast
circular DNA (chloroplast DNA), 70s ribosomes, many enzymes, starch granules and lipid droplets
Functions of central vacuole
• Stores water and other materials such as sugars, ions and pigments.
• Maintains water balance of the cell
• Gives turgidity and support to cell.
• Produce colours in some plants with sap pigments
• Stores soluble substances needed for cellular activities.
Pectins
(magnesium and calcium pectate)
pits through which cytoplasm of adjoining cells join through
plasmodesmata.
Types of cell junctions and places they are found in
Tight junctions - skin epithelium
Desomosomes/ Anchor junctions - Muscle tissue
Gap junctions/ Communicating junctions - heart muscles and animal embryo
Components of the ECM
glycoproteins and other carbohydrates containing molecules secreted by the cells
Most abundant glycoprotein in the ECM
Collagen
Functions of ECM
• Forms a protective layer over the cell surface
• Linking extra cellular matrix and cytoskeleton.
• Influences the cell behavior by Involving in the mechanical and chemical signaling.
Functions of cell wall
Protection and support
Allows development of turgidity when water enters the cell
Prevents bursting of the cell during turgidity
Component of apoplast pathway
Maintains shape of the cell
Limits and control cell growth
Hold the plant up against the force of gravity
The collagen fibers are embedded
In a network woven out of proteoglycan
Collagen fibers are connected to the integral proteins of the plasma membrane through
Fibronectin
Who helped bring the abiotic (nonliving) synthesis of organic compounds into the context of evolution
Stanley Miller
Thiol
HS
Most of the glucose monomers in starch are linked by
1-4 glycosidic bonds
Differences between glucose monomers of starch and cellulose
Glucose monomers of starch are in the alpha configuration while in cellulose it is in beta configuration
Phospho-di-ester bonds are formed between
the OH group of the phosphate group and the OH attached to the 3rd carbon of the pentose sugar group
Who found out the amino acid sequence of proteins
Frederick Sanger
What are chaperone proteins
Proteins that assist the conformational folding or unfolding of large proteins or macromolecular protein complexes
Steroids
Characterised by hydrocarbon skeletons consisting of 4 fused rings
Cholesterol is synthesised in the liver
Diseases caused by the accumulation of misfolded proteins
Cystic fibrosis
Mad-cow disease
Alzheimer’s
Parkinsons
Role of microtubules
Maintenance of cell shape
Organelle, chromosome movement
Cell motility as in cilia and flagella
Role of actin filaments
Maintenance of cell shape
Cell motility as in pseudopodia
Cytoplasmic streaming
Muscle contraction
Cleavage furrow formation
Intermediate filaments
Maintenance of cell shape
Anchorage of nucleus and other cell organelles
Formation of nuclear lamina