chemical analysis of urine 2 Flashcards
early indicator of liver disease
a. protein
b. glucose
c. bilirubin
bilirubin
legal’s test
a. bilirubin
b. ketones
c. glucose
ketones
principle
- legal’s test or sodium nitroprusside reaction
double sequential enzymatic reaction
a. glucose
b. ketones
c. bilirubin
glucose
pseudoperoxidase activity of heme
a. bilirubin
b. urobilirubin
c. blood
blood
diazo reaction
a. bilirubin
b. urobilirubin
c. blood
bilirubin
what kind of bilirubin is water soluble and can detect in urine
a. b1/ uncon
b. b2 / conjugated
conjugated/ b2
its reagent strip is difficult to interpret because it is easily influenced by other pigments present in urine
a. blood
b. bilirubin
c. ketones
bilirubin
what is the reaction in bilirubin reagent strip on highly exposure to light
a. false pos
b. false neg
false neg
what is the reaction in bilirubin reagent strip on indican
a. false pos
b. false neg
false pos
normal adult urine contains bilirubin only
a. <10mg/dl
b. 0,2mg/dl
c. 5mg/ml
0.2 mg/dl
confirmatory test for bilirubin
a. bloodheim’s precipitation
b. ictotest
c. acetest
ictotest
- confirmatory test for bilirubin
bloodheim’s test - hemoglobinuria vs. myoglobinuria
actest
- ketones
it is used to diagnosed the pre hepatic from hepatic
a. urobilinogen
b. ictotest
c. bilirubin
urobilinogen
how many urobilinogen can be found in normal urine
a. 0.2 mg/dl
b. <10mg/dl
c. 1 mg/dl
1 mg/dl - urobilinogen
0.2 mg/dl - bilirubin
how many ehrlich unit is 1mg/dl
a. 0.1
b. 1
c. 0.001
ratio of mg/dl : ehrlich unit
1:1
ans: b
preferred sample for urobilinogen
a. random urine
b. afternoon urine
c. first morning
afternoon urine (2pm-4pm)
What would be the result of urobilinogen measurements if the reagent strip is performed at a higher temperature?
a. false decreased
b. false increased
false increase
the sensitivity of the ehrlich reaction INCREASES WITH TEMPERATURE and testing should be performed at room temperature
watson- schwartz test
a. urobilinogen
b. bilirubin
c. blood
urobinogen
watson- schwartz test
soluble in both butanol and chloroform
a. urobilinogen
b. porphobilinogen
c. ehrlich reactive
urobilinogen - soluble in BOTH butanol and chloroform
watson- schwartz test
soluble in butanol only
a. urobilinogen
b. porphobilinogen
c. ehrlich reactive
ehrilich - soluble in butanol only
porpho - insoluble in both
uro - soluble in both
rapid screening test for porphobilinogen
a. bloodheim’s test
b. hoesch test
c. ictotest
hoesch test/ inverse ehrlich reaction
urine bilirubin : +/-
urine urobilinogen : +++
a. pre hepatic jaundice
b. hepatic jaundice
c. post hepatic jaundice
urine bilirubin : +/-
urine urobilinogen : +++
HEPATIC JANDICE ( liver damge) increase both conju and uncoju
urine bilirubin : negative
urine urobilinogen : +++
a. pre hepatic jaundice
b. hepatic jaundice
c. post hepatic jaundice
urine bilirubin : negative
urine urobilinogen : +++
pre hepatic jaundice (hemolytic disease)
b1
urine bilirubin : +++
urine urobilinogen : normal
a. pre hepatic jaundice
b. hepatic jaundice
c. post hepatic jaundice
post hepatic
b2 increased( bile duct obstruction)
principle: greiss reaction
a. nitrite
b. leukocyte
c. urobilinogen
niitrite
