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Biol 4880 > Chapter 8 Enzymes > Flashcards

Chapter 8 Enzymes Flashcards

1
Q

Gaucher Disease caused by ?

A

lack glucocerebrosidase

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2
Q

enzymes increase ?, but do not affect ?

A

reaction rate, equilbrium

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3
Q

enzymes stabilize ?

A

transition state

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4
Q

Two models of substrate binding

A

induced fit , lock and key

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5
Q

reaction velocity formula

A

v=[ES]*kcat

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6
Q

steady state assumption

A

[ES] stays unchanged

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7
Q

M-M equation

A

V= ( Vmax * [S] ) / ( [S] + Km )

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8
Q

When [S] = Km, the M-M equation is simplified to ?

A

V= Vmax/2

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9
Q

Km is the concentration where ?

A

the velecity of the reaction is half of the maximum velocity

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10
Q

LB plots ? vs ?

A

1/[S] vs 1/V

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11
Q

catalytic efficiency formula

A

V=(kcat/Km)[E][S]

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12
Q

Three types of substrate addition

A

ordered sequential, random sequential, double displacement

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13
Q

Allosteric enzymes (do/do not) obey MM kinetics

A

do not

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14
Q

Three types of of reversible enzyme inhibition

A

competitive, uncompetitive, noncompetitive

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15
Q

Competitive inhibitor

A

replaces substrate at active site

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16
Q

DHFR is competitively inhibited by ?

A

methotrexate

17
Q

x-intercept of LB plot

A

-1/Km

18
Q

y-intercept of LB plot

A

1/Vmax

19
Q

affect of competitive inhibitors on LB plot

A

changes x-intercept

20
Q

uncompetitive inhibitors bind to ?

A

ES complex

21
Q

affect of uncompetitive inhibitors on LB plot

A

changes both x and y intercept, slope remains the same

22
Q

noncompetitive inhibitors bind to ?

A

site other than the active site

23
Q

affect of noncompetitive inhibitors on LB plot

A

changes y-intercept

24
Q

hydrolases

A

hydrolysis reactions, transfer of functional groups to water

25
Q

lyases

A

addition or removal of groups to form double bonds

26
Q

isomerases

A

isomerization, intramolecular group transfers

27
Q

ligases

A

bind two substrates at the cost of ATP

28
Q

holoenzyme

A

apoenzyme + cofactor

29
Q

bohr effect refers to?

A

more oxygen released from Hb at lower pH

30
Q

a measure of the affinity of the enzyme for the substrate

A

Km

31
Q

lower Km means (lower/higher) affinity

A

higher

32
Q

fetal hemoglobin has higher affinity to O2 than maternal because

A

fetal Hb has lower affinity for BPG

33
Q

Kcat

A

turnover rate

34
Q

acyl group binds to ?

A

S

35
Q

process to produce chymotrypsin is an example of?

A

proteolytic activation

36
Q

nucleoside

A

sugar + base

37
Q

number of pyrrole rings in one heme

A

4

38
Q

When oxygen binds, Fe is drawn (into/out of) plane of porphyrin ring

A

into

Biol 4880 (13 decks)

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