Chapter 8 Enzymes Flashcards
Gaucher Disease caused by ?
lack glucocerebrosidase
enzymes increase ?, but do not affect ?
reaction rate, equilbrium
enzymes stabilize ?
transition state
Two models of substrate binding
induced fit , lock and key
reaction velocity formula
v=[ES]*kcat
steady state assumption
[ES] stays unchanged
M-M equation
V= ( Vmax * [S] ) / ( [S] + Km )
When [S] = Km, the M-M equation is simplified to ?
V= Vmax/2
Km is the concentration where ?
the velecity of the reaction is half of the maximum velocity
LB plots ? vs ?
1/[S] vs 1/V
catalytic efficiency formula
V=(kcat/Km)[E][S]
Three types of substrate addition
ordered sequential, random sequential, double displacement
Allosteric enzymes (do/do not) obey MM kinetics
do not
Three types of of reversible enzyme inhibition
competitive, uncompetitive, noncompetitive
Competitive inhibitor
replaces substrate at active site
DHFR is competitively inhibited by ?
methotrexate
x-intercept of LB plot
-1/Km
y-intercept of LB plot
1/Vmax
affect of competitive inhibitors on LB plot
changes x-intercept
uncompetitive inhibitors bind to ?
ES complex
affect of uncompetitive inhibitors on LB plot
changes both x and y intercept, slope remains the same
noncompetitive inhibitors bind to ?
site other than the active site
affect of noncompetitive inhibitors on LB plot
changes y-intercept
hydrolases
hydrolysis reactions, transfer of functional groups to water
