Chapter 4 DNA, RNA

Question 1

retrovirus mechanism

Answer 1

RNA virus uses reverse transcriptase to create DNA that is incorporated into host DNA

Question 2

nucleosides consist of ? (2)

Answer 2

sugar and base

Question 3

sugar and base connected by ?

Answer 3

glycosidic linkage

Question 4

adenine DNA nucleoside name

Answer 4

deoxyadenosine

Question 5

uracil RNA nucleoside name

Answer 5

uridine

Question 6

guanine DNA ribonucleotide name

Answer 6

deoxyguanosinemonophosphate

Question 7

rotation around the glycosidic in a nucleoside is sterically hindered. (syn/anti) preferred.

Answer 7

anti

Question 8

chargaff’s rule

Answer 8

ratio of A:T and C:G is 1

Question 9

intercalating agents

Answer 9

chemical that can insert itself between the stacked bases at the centre of the DNA double helix

Question 10

mutagens

Answer 10

change genetic material, usually DNA

Question 11

watson and crick suggested DNA replication would be ?. In other words, replicated DNA would contain one original strand.

Answer 11

semi-conservative

Question 12

? and ? demonstrated the semi-conservative nature of DNA

Answer 12

meselson and stahl

Question 13

single stranded nucleic acids absorb (more/less) UV light because..

Answer 13

more. base resonance absorbs light and are more exposed in single strand

Question 14

melting temperature formula

Answer 14

Tm= 4(G+C) + 2(A+T) based on one strand

Question 15

more complex DNA takes (more/less) time to re associate after being dissociated

Answer 15

more

Question 16

EDTA is a ? agent. Causes easier dissociation of double strands.

Answer 16

chelating

Question 17

DNA denaturation is (1st/2nd) order

Answer 17

2nd

Question 18

DNA renaturation us (1st/2nd) order

Answer 18

1st