Chapter 18 ETC Flashcards
four complexes of ETC
NADH-Q oxidoreducatase, succinate-Q reductase, Q-cytochrome c oxidoreductase, cytochrome c oxidase
three proton pumps of ETC
NADH-Q oxidoreducatase, Q-cytochrome c oxidoreductase, cytochrome c oxidase
complex 1
NADH-Q oxidoreducatase
complex 2
succinate-Q reductase
complex 3
Q-cytochrome c oxidoreductase
complex 4
cytochrome c oxidase
complex 1 function
transfer electrons from NADH to Q
complex 2 function
transfer electrons from FADH2 to Q
complex 3 function
transfer electrons from Q to cytochrome c
complex 4 function
transfer of electrons from cytochrome c to O2
electrons passed from ? to ? electrode potential
low to high
high/low electrode potential is indicative of high affinity for electrons
high
net reaction of complex 3
QH2 + 2cytC (ox) + 2H+ (matrix) goes to Q + 2cytC (red) + 4H+ (cytoplasm)
ETC pumps protons into ?
intermembrane space
complex 4 produces water in ?
matrix
superoxide dismutase reaction
2O2- + 2H+ goes to O2 + H2O2
catalase reaction
2H2O2 goes to O2 + 2H2O
ATPsynthase structure
one gamma subunit, three alpha, three beta
gamma subunit induces beta subunit into these three conformations
open, loose, tight
open conformation of ATPsynthase
allows ATP to be released and allows ADP and Pi to enter
loose conformation of ATPsynthase
holds ADP and Pi in place
tight conformation of ATPsynthase
forces ATP synthesis from ADP and Pi
number of protons transported across ATPsynthase per turn
12
number of ATP made per turn
3
number of proton transfers required to make one ATP
4
ATP/O ratio for ATP synthesis
2.5 (10/4). 4 protons required for ATP synthesis. Ten protons pumped per NADH to reduce one oxygen.
protons pumped per NADH
10
G3P shuttle function
Allows NADH produced in glycolysis to be incorporated in ETC
G3P shuttle net reaction
NADH + H+ +EFAD goes to NAD+ + EFADH2. EFADH2 transfers electrons to Q.
malate-aspartate shuttle function
electrons transported from NADH to malate, malate moves into matrix from cytoplasm, malate gives electrons to NAD+. Glutamate is transported across membrane to cytoplasm by converting to aspartate.
protons pumped per FADH2
6
glycolysis yield
2 ATP, 2NADH
pyruvate dehydrogenase yield
2 NADH
CAC yield
2ATP, 6NADH, 2FADH2
NADH and FADH2 yield from glycolysis and CAC for one glucose
10NADH and 2FADH2
ATP yield from glycolysis and CAC for one glucose
4ATP
10NADH pumps ? protons
100
2FADH2 pumps ? protons
12
112 protons pumped creates ? ATP
28
net ATP yield of one glucose molecule through glycolysis, CAC and ETC
32