Chapter 7 - Protein Flashcards

1
Q

protein

A

very large molecules composed of one or more chains of amino acids

molecules critical for muscle+bone structure, most biochemical reactions

4 different types: primary, secondary, tertiary, and quaternary

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2
Q

amino acids

A

very small molecules, building blocks of proteins

dipeptides, tripeptides, and polypeptides

hundreds of amino acids are found in nature, but only 20 are used by the human body

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3
Q

dipeptide

A

peptide composed of 2 amino acids

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4
Q

tripeptides

A

peptide composed of 3 amino acids

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5
Q

titin

A

A giant structural protein in the human body that helps provide structure to sarcomeres (the basic contractile unit of muscle fiber) in muscle tissue.

27,000 amino acids - one of the largest and most complex

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6
Q

how many structures of proteins are there?

A

4

primary, secondary, tertiary, and quaternary

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7
Q

protein structure

primary structure

A

the order of amino acids in peptide molecule

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8
Q

protein structure

secondary structure

A

The primary shape of a protein, usually classified as a
1. beta-sheet, or
2. alpha-helix.

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9
Q

protein structure

tertiary structure

A

The full, three-dimensional structure of a single chain of amino acids

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10
Q

protein structure

quaternary structure

A

The structure when two or more tertiary structures interact

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11
Q

types of protein

A
  1. fibrous
  2. globular
  3. membrane
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12
Q

protein type

fibrous proteins

A

These proteins form muscle fibers, tendons, connective tissue, and bones.

  1. collagen
  2. titin
  3. elastin
  4. myosin
  5. actin
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13
Q

protein type

globular proteins

A

These proteins are water-soluble, shaped like a globe or sphere and act as transport proteins, enzymes, and hormones

  1. hemoglobin
  2. albumin
  3. fibrin
  4. immunoglobulin A
  5. myoglobin
  6. insulin
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14
Q

protein type

membrane proteins

A

integral component of cell membranes

found in all cells and separates the interior of the cell from the outside environment

signaling proteins:
1. integral
2. peripheral
3. lipid-anchored

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15
Q

essential amino acids

A

cannot be synthesized by the body, must be consumed through the diet
1. histidine
2. lysine
3. methionine
4. phenylalanine
5. thrreonine
6. tryptophan
7. branched-chain amino acids
8. isoleucine
9. leucine
10. valine

p i t t h m b l l v

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16
Q

non-essential amino acids

A

Amino acids that the human body is capable of manufacturing to a large enough degree that they are not required to be consumed through the diet
1. alanine
2. asparagine
3. aspartic acid
4. glutamic acid
5. serine
6. Arginine
7. Cysteine
8. Glutamine
9. Glycine
10. Proline
11. Tyrosine

c a a a a g g g s t p

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17
Q

4 steps of protein metabolism

A
  1. chewing
  2. stomach acid
  3. small intestine
  4. bloodstream
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18
Q

factors affecting protein absorption

A

internal factors: related to the human body
external factors: related to the protein source itself

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19
Q

factors affecting protein absorption

external factors

A
  1. state of protein (liquid or solid)
  2. cooked or not
  3. complexity of the protein type/structure
  4. whether it is consumed in isolation
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20
Q

external absorption factors

liquid proteins

A

Liquid protein sources (e.g., whey protein or dairy) are often more efficiently absorbed when compared to solid sources

This is true in part because liquid proteins require little to no mechanical digestion and are more easily digested enzymatically by peptidases in the stomach

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21
Q

external absorption factors

food processing

A

around 90% of cooked egg protein is absorbed while only ~50% of raw egg protein is absorbed

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22
Q

external absorption factors

complexity of protein structure

A

proteins that contain smaller peptides, such as di- or tripeptides, are often absorbed slightly faster than proteins that have longer peptide chains

Whey protein is a good example of this, especially in the context of sports nutrition. There is some evidence that consuming a hydrolyzed whey protein, which is enzymatically pre-digested, is absorbed slightly faster than a fully intact whey protein

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23
Q

external absorption factors

consuming protein in isolation

A

consuming protein in isolation appears to be more beneficial for acutely maximizing absorption when compared to mixed meals

There are some tools that the Sports Nutrition Coach can use to help determine how digestible a protein source is and how much that protein source contributes to the free amino acid pool

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24
Q

internal absorption factors

stomach enzymes

A

the amount of peptidases (peptide digesting enzymes) present in the gastrointestinal tract varies from person to person

the less peptidases present, the less efficient someone will digest and absorb protein

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25
# internal absorption factors stomach acid
stomach acid also affects protein digestion and absorption ## Footnote individuals with less stomach acid often absorb less of the protein they consume
26
# internal absorption factors volume of protein consumed
the efficiency of digestion and absorption decreases as the volume and amount of protein increases ## Footnote **For example, if someone consumes ~10 grams of protein, they will absorb a higher percentage of those 10 grams than they would if they were to consume ~100 grams of protein**
27
Digestion, Absorption, and the Athlete
The digestive and absorptive properties of various proteins can have important impacts on athletes For example, the digestive properties of proteins can influence the anabolic response to protein both during exercise and at rest. These proteins are often classified as fast or slow based on digestion and absorption The majority of research into fast and slow protein compares whey, casein, and soy (the most commonly consumed isolated protein). Whey and soy are considered to be fast proteins, whereas casein is slow. Another factor to consider when choosing the type of protein to consume is the amino acid content, specifically leucine. This amino acid acts as a signaling molecule that stimulates protein synthetic pathways such as mTOR. Whey protein contains more leucine than casein or soy; however, supplementation can often contain additional leucine, which promotes anabolic response post-exercise
28
plant-based athletes
plant-based diets tend to consume less protein overall, and because plant-based protein sources are incomplete, they can increase the risk of amino acid deficiencies Plant-based diets are often found to be lower in leucine, lysine, methionine, isoleucine, threonine, and tryptophan
29
complimentary proteins
A culmination of proteins that, on their own, are missing some essential amino acids, **but when combined have a complete amino acid profile**
30
free amino acid pool
The amino acids present in this pool are then transported around the body, used where required, and are constantly incorporated into protein structures and broken back down to amino acids ## Footnote the body contributes 400 g daily to this pool
31
# protein breakdown and synthesis protein turnover
The balance of protein synthesis and protein degradation ## Footnote the half-life of proteins can range from minutes to weeks, depending upon the function of the specific protein
32
# protein breakdown and synthesis protein synthesis
process of making protein ## Footnote Synthesis is the creation of larger protein structures from individual amino acids
33
# protein breakdown and synthesis protein degradation
breaking down protein
34
hypertrophy
the enlargement of a tissue or organ ## Footnote in the context of fitness, it is often used to describe the enlargement of skeletal muscle
35
atrophy
The wasting away or decrease in size of body tissue
36
nitrogen balance
A measure of nitrogen input versus nitrogen output ## Footnote Historically, one of the ways to assess protein balance (ratio of protein synthesis to protein breakdown) was using a method known as nitrogen balance. This method examined the rate at which dietary nitrogen was entering the body through protein consumption and the rate at which it was leaving the body through urine. This method was used for several decades, but recent methodologies have been developed that are much more accurate, such as indicator amino acid oxidation tests
37
Protein as an Energy Source
Carbohydrates and fats serve as the primary fuel sources in the human body; however, during periods of low energy availability or low nutrient availability, protein—specifically amino acids—can be utilized to provide energy
38
# Protein as an Energy Source three pathways for amino acid energy
1. First, some amino acids can be converted into **pyruvate**, which eventually becomes Acetyl-CoA that then enters into the Krebs cycle 2. amino acids can be converted into α-ketoglutarate or **fumarate**, which are intermediaries in the Krebs cycle 3. amino acids are used as substrates for creating more **glucose** through the process of gluconeogenesis, which requires lactate, glycerol, and amino acids
39
how much protein to eat per meal
more protein will elicit a greater muscle protein synthesis response | 20 - 40 g is good ## Footnote there are diminishing returns as the amount of protein synthesis goes up
40
timing of dietary protein
based on the current literature, it appears that consuming protein post-workout is important, but the window may be as long as 4-6 hours, and that daily protein intake is likely the most important factor
41
# protein myths kidney function
dietary protein DOES NOT cause kidney damange ## Footnote **does not extend to those with established kidney disease
42
what 6 functions does protein serve in the body?
1. tissue remodeling (protein synthesis and degradation) 2. enzymes + cellular transporters 3. cell signaling 4. fluid balance 5. hormones and neurotransmitters 6. immune system function
43
# protein myths BCAAs
they DO NOT increase muscle protein synthesis and DO NOT increase weight loss ## Footnote valine is an important BCAA
44
# protein myth protein and bone health
protein DOES NOT cause weak and brittle bones ## Footnote A series of studies demonstrated that low protein intake results in less calcium absorption than high-protein diets, and that higher protein intake is associated with greater bone mineral density than lower protein intake
45
# protein myths soy protein
because soy is lower in the essential amino acid leucine, it DOES NOT produce as great of muscle protein synthesis response as whey protein (which has more leucine)
46
# protein myths protein and the vegan athlete
Athletes who adopt a plant-based diet often consume less protein than their omnivorous counterparts it has been estimated that athletes who follow a plant-based diet have an increased protein requirement of 20 or more grams per day than athletes who follow an omnivorous diet
47
mTOR
mTOR, which is known as the mammalian target of rapamycin, is an enzyme that is involved in the cell signaling processes that **increase muscle protein synthesis** ## Footnote **leucine** provides one of the most potent signals for activating it.
48
# protein function bodily tissue
proteins are an important component of other bodily tissues Protein is important for bone tissue, connective tissue (e.g., tendons and ligaments), and organ tissue
49
# protein function enzymes and cellular transporters
critical in biochemical reactions through their roles as enzymes and cellular transporters, moving nutrients, hormones, electrolytes, and other molecules throughout the body
50
# protein function cell signaling
Proteins are critical in these cellular signaling processes in that most of the nodes in these signals are made of proteins
51
# protein function fluid balance
Protein plays an important role in ensuring that fluids are correctly distributed across these three compartments. When proteins are located within circulation, they exert what is known as **colloid-osmotic pressure**, which means that they attract water, allowing the body to hold an adequate amount of fluid within circulation
52
# protein function hormones and neurotransmitters
hormones and neurotransmitters are made of amino acids
53
oxytocin
produced by the hypothalamus and secreted by the pituitary gland that acts as a neurotransmitter and is **involved in childbirth and breast-feeding.**
54
cytokines
A broad category of molecules, often proteins, involved in cell signaling.
55
chemokines
A class of proteins that stimulate the directional movement of other cells (leukocytes, epithelial cells, etc).
56
suggested average protein intake for average adult and average athlete
adult: 0.8 g/kg athlete: 1.6-2.2 g/kg
57
# protein requirements the endurance athlete
training-induced adaptations such as mitochondrial recovery and tendon and ligament remodeling **1.6 - 1.7 g/kg represents the minimal threshold of protein intake to prevent degredation of muscle mas**s (it's not necessarily the optimal amount)
58
# protein requirements the strength and power athlete
1.6 - 2.0 g/kg
59
# protein requirements team sport athlete
1.4 g/kg daily soccer, rugby, basketball, etc.
60
Protein Requirements by Training Volume
as training volume increases, tissue turnover increases, which requires more protein
61
effect of age on protein requirements
It has been proposed that 1.6–2.0 g per kg body weight per day should be recommended to optimize muscle protein synthesis in older athletes, especially as training and competition cause damage to contractile and connective tissue there is a phenomenon known as anabolic resistance present among older individuals, in which the anabolic response to amino acids is impaired among older adults (~65 years of age and older)
62
Effect of Biological Sex on Protein Requirements
there are similar protein requirements between sexes
63
In addition to amino acids, what other substrate can be used to make glucose from protein?
glycerol
64
Protein makes up what portion of bone tissue?
1/3
65
Most amino acids are absorbed in the intestine in what form?
dipeptides
66
What two amino acids make up almost all of collagen proteins?
glycine and proline
67
Which amino acid is considered conditionally essential?
glycine
68
Athletes recovering from injury most likely require protein intake in what range?
2 - 2.4 g
69
On average, vegan athletes likely require how many more grams of protein per day than omnivorous athletes?
20 g