Chapter 6 pt.2

Question 1

used in microbiology lab to culture bacteria

Answer 1

media / medium (singular)

Question 2

media prepared in lab

Answer 2

artificial or synthetic media

Question 3

broth or solid medium containing a rich supply of special nutrients that promote the growth of fastidious organisms

Answer 3

enriched medium

Question 4

heated blood / heated BAP

Answer 4

chocolate agar

Question 5

has added inhibitors that discourage growth of certain organisms while allowing the growth of DESIRED organism

Answer 5

selective medium

Question 6

allows gram (-) to grow but inhibits gram (+) to grow (selective and differential medium)

Answer 6

MAC Conkey Agar

Question 7

permits the differentiation of organisms that grow on medium

Answer 7

differential medium

Question 8

types of hemolysis

Answer 8

alpha = incomplete (greenish brown)
beta = complete (colorless)
gamma = no hemolysis

Question 9

medium that can support obligate aerobes and anaerobes

Answer 9

Thioglycollate broth (THIO broth)

Question 10

fastidious organisms require ???

Answer 10

blood

Question 11

to prevent contamination

Answer 11

aseptic technique

Question 12

unwanted organisms

Answer 12

contaminants

Question 13

will maintain ambient environment (controlled temp and moisture)

Answer 13

incubator

Question 14

incubator that contains 5-10% of CO2

Answer 14

CO2 incubator

Question 15

incubator that contains room air

Answer 15

non-CO2 incubator

Question 16

incubator that is devoid of oxygen ; for obligate anaerobes

Answer 16

anaerobic incubator

Question 17

used to determine growth by measuring the turbidity of the medium

Answer 17

spectrophotometer

Question 18

used to determine number of viable bacteria in a LIQUID sample

Answer 18

viable plate count

Question 19

may be determined by growing a pure culture of the organism

Answer 19

population growth curve

Question 20

biglang taas ng growth of bacterias

• best time to perform antibacterial eme
• produces primary, secondary metabolites (antibiotics)

Answer 20

log (exponential) phase

Question 21

made up of young viable cells

- bacteria is adjusting

Answer 21

lag phase

Question 22

to prevent this phase, transfer bacteria in fresh culture medium

Answer 22

death phase

Question 23

plateau (no. of living = no. of death)

- bc of depletion of nutrients and accumulation of metabolic waste product

Answer 23

stationary phase

Question 24

microbes that can survive and multiply only within living cells
- virus, rickettsia, chlamydia

Answer 24

obligate intracellular pathogens

Question 25

popular live cell; live tissue culture

Answer 25

HeLa cells (Henrietta Lacks)

Question 26

ex of culture media for fungi

Answer 26

brain heart infusion (BHI) agar BHI with blood SDA

Question 27

protozoa that can be cultured in diamond serum medium

Answer 27

Trichomonas vaginalis

Question 28

complete destruction of all microbes including cells, spores, and viruses

Answer 28

sterilization

Question 29

steam under pressure; 121deg for 15 PSI for 15-20 mins | - destroys vegetative microorganisms, bacterial endospores, and viruses

Answer 29

autoclave

Question 30

destruction or removal of pathogens from non-living objects

Answer 30

disinfection

Question 31

chemical substances that eliminate pathogens on inanimate objects

Answer 31

disinfectants

Question 32

solutions used to disinfect skin and other living tissues

Answer 32

antiseptics

Question 33

chemicals that kill microbes

Answer 33

germicidal, biocidal, and microbicidal agents

Question 34

specifically kill bacteria but not necessarily bacterial endospores

Answer 34

bactericidal agents

Question 35

kill bacterial endospores

Answer 35

sporicidal agents

Question 36

kill fungi and fugal spores

Answer 36

fungicidal agents

Question 37

kill algae

Answer 37

algicidal

Question 38

destroy viruses

Answer 38

viricidal

Question 39

drug or chemical that inhibits growth and reproduction of microbes

Answer 39

microbistatic agent

Question 40

process of dehydration, drying, and deep freezing

Answer 40

lyphilization

Question 41

presence of pathogens in blood or tissues

Answer 41

sepsis

Question 42

absence of pathogens

Answer 42

asepsis

Question 43

prevention of infection

Answer 43

antisepsis

Question 44

lowest temp that will kill all of the organisms within a specified time

Answer 44

thermal death point (TDP)

Question 45

many dried microorganisms remain viable but can't reproduce

Answer 45

desiccation

Question 46

not killed but metabolic activities are slowed

Answer 46

cold

Question 47

UV lamp is useful for reducing the number of microbes in the air

Answer 47

radiation

Question 48

used in hospitals and medical clinics to clean equipment

Answer 48

ultrasonic waves

Question 49

used to separate cells from liquids or gases (for bacteria only)

Answer 49

filters

Question 50

use of chemical agents to inhibit the growth of pathogens, either temporarily or permanently

Answer 50

chemical disinfection