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Microbiology and Parasitology > Chapter 6 pt.2 > Flashcards

Chapter 6 pt.2 Flashcards

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Biology 101 flashcards
1
Q

used in microbiology lab to culture bacteria

A

media / medium (singular)

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2
Q

media prepared in lab

A

artificial or synthetic media

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3
Q

broth or solid medium containing a rich supply of special nutrients that promote the growth of fastidious organisms

A

enriched medium

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4
Q

heated blood / heated BAP

A

chocolate agar

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5
Q

has added inhibitors that discourage growth of certain organisms while allowing the growth of DESIRED organism

A

selective medium

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6
Q

allows gram (-) to grow but inhibits gram (+) to grow (selective and differential medium)

A

MAC Conkey Agar

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7
Q

permits the differentiation of organisms that grow on medium

A

differential medium

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8
Q

types of hemolysis

A 
alpha = incomplete (greenish brown)
beta = complete (colorless)
gamma = no hemolysis
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9
Q

medium that can support obligate aerobes and anaerobes

A

Thioglycollate broth (THIO broth)

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10
Q

fastidious organisms require ???

A

blood

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11
Q

to prevent contamination

A

aseptic technique

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12
Q

unwanted organisms

A

contaminants

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13
Q

will maintain ambient environment (controlled temp and moisture)

A

incubator

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14
Q

incubator that contains 5-10% of CO2

A

CO2 incubator

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15
Q

incubator that contains room air

A

non-CO2 incubator

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16
Q

incubator that is devoid of oxygen ; for obligate anaerobes

A

anaerobic incubator

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17
Q

used to determine growth by measuring the turbidity of the medium

A

spectrophotometer

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18
Q

used to determine number of viable bacteria in a LIQUID sample

A

viable plate count

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19
Q

may be determined by growing a pure culture of the organism

A

population growth curve

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20
Q

biglang taas ng growth of bacterias

  • best time to perform antibacterial eme
  • produces primary, secondary metabolites (antibiotics)
A

log (exponential) phase

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21
Q

made up of young viable cells

- bacteria is adjusting

A

lag phase

22
Q

to prevent this phase, transfer bacteria in fresh culture medium

A

death phase

23
Q

plateau (no. of living = no. of death)

- bc of depletion of nutrients and accumulation of metabolic waste product

A

stationary phase

24
Q

microbes that can survive and multiply only within living cells
- virus, rickettsia, chlamydia

A

obligate intracellular pathogens

25
Q

popular live cell; live tissue culture

A

HeLa cells (Henrietta Lacks)

26
Q

ex of culture media for fungi

A

brain heart infusion (BHI) agar
BHI with blood
SDA

27
Q

protozoa that can be cultured in diamond serum medium

A

Trichomonas vaginalis

28
Q

complete destruction of all microbes including cells, spores, and viruses

A

sterilization

29
Q

steam under pressure; 121deg for 15 PSI for 15-20 mins

- destroys vegetative microorganisms, bacterial endospores, and viruses

A

autoclave

30
Q

destruction or removal of pathogens from non-living objects

A

disinfection

31
Q

chemical substances that eliminate pathogens on inanimate objects

A

disinfectants

32
Q

solutions used to disinfect skin and other living tissues

A

antiseptics

33
Q

chemicals that kill microbes

A

germicidal, biocidal, and microbicidal agents

34
Q

specifically kill bacteria but not necessarily bacterial endospores

A

bactericidal agents

35
Q

kill bacterial endospores

A

sporicidal agents

36
Q

kill fungi and fugal spores

A

fungicidal agents

37
Q

kill algae

A

algicidal

38
Q

destroy viruses

A

viricidal

39
Q

drug or chemical that inhibits growth and reproduction of microbes

A

microbistatic agent

40
Q

process of dehydration, drying, and deep freezing

A

lyphilization

41
Q

presence of pathogens in blood or tissues

A

sepsis

42
Q

absence of pathogens

A

asepsis

43
Q

prevention of infection

A

antisepsis

44
Q

lowest temp that will kill all of the organisms within a specified time

A

thermal death point (TDP)

45
Q

many dried microorganisms remain viable but can’t reproduce

A

desiccation

46
Q

not killed but metabolic activities are slowed

A

cold

47
Q

UV lamp is useful for reducing the number of microbes in the air

A

radiation

48
Q

used in hospitals and medical clinics to clean equipment

A

ultrasonic waves

49
Q

used to separate cells from liquids or gases (for bacteria only)

A

filters

50
Q

use of chemical agents to inhibit the growth of pathogens, either temporarily or permanently

A

chemical disinfection

Microbiology and Parasitology (10 decks)

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