Chapter 5b - Notes Flashcards
very important for initial binding of RNA pol
σ factor sigma
turn transcription off
silencer
Series of Events of Signal towards RNA polymerase (transcription on)
- Enhancer
- Activator
- Coactivator
- Basal Factors
- RNA Polymerase
Series of Events of Signal towards RNA polymerase (transcription off)
- Repressor
- Silencer
- Activator
- Coactivator
- Basal Factors
- RNA Polymerase
Eukaryotic Promoter site
- -25 TATA box (Goldberg–Hogness box)
What dissociates after RNA polymerase successfully binds to DNA strand
σ factor sigma
- sequence of RNA in bacteria upstream of the terminator region which serves as a binding site for the protein known as rho factor
- This sequence is necessary in rho-dependent termination of DNA transcription in bacteria
Rut- Rho utilization
Initiation Factors of Transcription in Eukaryotes
- TBP
2.TF2D, TF2A, TF2B - TF2E, TF2H, TF2F
- RNA Pol.
cut mRNA during transcription
CPF
how many poly As are added during tailing
100-200 residues
section of a DNA or RNA molecule that codes for a specific polypeptide in protein synthesis
cistron
mRNA that encodes several proteins and is characteristic of many bacterial and chloroplast mRNAs
Polycistronic mRNA
highly conserved sequence in the tRNA
CCA
Structure of tRNA
- Acceptor stem
- D-arm
- anticodon
- Anticodon arm
- Variable arm
- T-arm
- Discriminator base
- The end of a tRNA molecule to which an amino acid becomes bound
- It contains both the 5’ and 3’ ends of the tRNA
acceptor stem