Chapter 5b - Notes Flashcards
very important for initial binding of RNA pol
σ factor sigma
turn transcription off
silencer
Series of Events of Signal towards RNA polymerase (transcription on)
- Enhancer
- Activator
- Coactivator
- Basal Factors
- RNA Polymerase
Series of Events of Signal towards RNA polymerase (transcription off)
- Repressor
- Silencer
- Activator
- Coactivator
- Basal Factors
- RNA Polymerase
Eukaryotic Promoter site
- -25 TATA box (Goldberg–Hogness box)
What dissociates after RNA polymerase successfully binds to DNA strand
σ factor sigma
- sequence of RNA in bacteria upstream of the terminator region which serves as a binding site for the protein known as rho factor
- This sequence is necessary in rho-dependent termination of DNA transcription in bacteria
Rut- Rho utilization
Initiation Factors of Transcription in Eukaryotes
- TBP
2.TF2D, TF2A, TF2B - TF2E, TF2H, TF2F
- RNA Pol.
cut mRNA during transcription
CPF
how many poly As are added during tailing
100-200 residues
section of a DNA or RNA molecule that codes for a specific polypeptide in protein synthesis
cistron
mRNA that encodes several proteins and is characteristic of many bacterial and chloroplast mRNAs
Polycistronic mRNA
highly conserved sequence in the tRNA
CCA
Structure of tRNA
- Acceptor stem
- D-arm
- anticodon
- Anticodon arm
- Variable arm
- T-arm
- Discriminator base
- The end of a tRNA molecule to which an amino acid becomes bound
- It contains both the 5’ and 3’ ends of the tRNA
acceptor stem
- recognition process is the primary purpose
- serves as a recognition site for the enzyme aminoacyl-tRNA synthetase.
D arm
three-nucleotide sequence found on tRNA that binds to the corresponding mRNA sequence
anticodon
site of the anticodon, which is complementary to the mRNA codon and determines which amino acids are carried
anticodon arm
helps in stabilizing tRNA
variable arm
specialized region on the tRNA molecule which acts as a special recognition site for the ribosome to form a tRNA-ribosome complex during protein biosynthesis or translation
T-arm
natural purine nucleoside that commonly occurs in transfer RNAs in humans
Inosine
ribonucleoprotein particle essential for the targeting of signal peptide-bearing proteins to the prokaryotic plasma membrane or the eukaryotic endoplasmic reticulum membrane for secretion or membrane insertion.
signal recognition particle (SRP)