CHAPTER 4: Lesson 15: Introduction to Genetic Engineering and Molecular Methods Flashcards

1
Q

is the deliberate modification of an organism’s genetic
information by directly changing its nucleic acid genome and is achieved by a group
of methods known as recombinant DNA technology.

A

Genetic engineering

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2
Q

a group of methods known as

A

recombinant DNA technology

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3
Q

is of value on basic research on gene structure and
function, production of useful proteins by novel methods, generation of transgenic
plants and animals, medical diagnosis and treatment or genome analysis by DNA
sequencing.

A

Genetic manipulation

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4
Q

The term has been coined to describe the
ethical problems that exist in modern genetics

A

genethics

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5
Q

are likely to increase in both
number and complexity as genetic engineering technology becomes more
sophisticated

A

genethics

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6
Q

example of advanced genetic engineering technology

A

development of Genetically Modified Organisms (GMOs).

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7
Q

The process of artificially
introducing foreign genes into organisms is termed

A

transfection

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8
Q

The process of artificially
introducing foreign genes into organisms is termed transfection, and the recombinant
organisms produced in this way are called

A

transgenic or Genetically Modified
organisms (GMOs)

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9
Q

Foreign genes have been inserted into a variety of microbes, plants, and
animals through

A

recombinant DNA techniques

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10
Q

One useful property of DNA

A

it readily
anneals (changes its binding properties in response to heating and cooling)

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11
Q

Exposure to temperatures _____________ causes DNA to become
temporarily denatured

A

just below boiling (90–95°C)

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12
Q

Exposure to temperatures just below boiling (90–95°C) causes DNA to become
_______.

A

temporarily denatured

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13
Q

When heating is followed by gradual cooling, two single DNA strands _________ by hydrogen bonds at complementary sites

A

rejoin (renature)

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14
Q

When heating is followed by gradual cooling, two single DNA strands rejoin (renature) by _________ at complementary sites

A

hydrogen bonds

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15
Q

is a necessary feature of the polymerase chain reaction (PCR) and nucleic acid probes

A

Annealing

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16
Q

Annealing is a necessary feature of the __________.

A

Polymerase Chain Reaction (PCR)
&
Nucleic Acid Probes

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17
Q

possessed by bacteria which allows them to recognize
specific regions of DNA for cleavage in producing DNA fragments to be used in
genetic engineering

A

Restriction enzymes

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18
Q

DNA sequences recognized by restriction enzymes are
predominantly __________.

A

palindromes

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19
Q

Inverted sequence repetitions

A

palindromes

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20
Q

Methods used to Size, Synthesize, and Sequence DNA

A

 Hybridization
 DNA sequencing
 Nucleic acid probes:

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21
Q

It is the process of combining two complementary single-stranded
DNA or RNA molecules and allowing them to form a single double-stranded
molecule through base pairing.

A

Hybridization

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22
Q

Types of hybridization

A
  1. Northern blot
  2. Southern blot
  3. Western blot
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23
Q

Hybridization of DNA to RNA, which
provides quantitative information about RNA synthesis.

A

Northern blot

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24
Q

Hybridization of DNA to DNA.

A

Southern blot

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25
This method is useful to detect specific DNA sequences in restriction fragments separated on gels. These blots can be used to detect overlapping restriction fragments.
Southern blot
26
Southern blot is used to....
detect overlapping restriction fragments
27
It is a technique used for detection of genes, in which antibodies are used to detect cloned genes by binding to their protein products.
Western blot
28
are used to detect cloned genes by binding to their protein products
antibodies
29
It shows gene structure that helps research workers to find out the structure of gene products.
DNA sequencing
30
DNA Sequencing Applications
■ Information obtained by DNA sequencing makes it possible to understand or alter the function of genes. ■ DNA sequence analysis demonstrates regulatory regions that control gene expression and genetic “hot spots” particularly susceptible to mutation. ■ Comparison of DNA sequences shows evolutionary relationships that provide a framework for definite classification of microorganisms including viruses. ■ Comparison of DNA sequences facilitates identification of conserved regions, which are useful for development of specific hybridization probes to detect microorganisms including viruses in clinical samples
31
are segments of DNA and RNA labeled with radioisotopes or enzymes that can hybridize to complementary nucleic acids with high degree of specificity.
Nucleic acid probes
32
The basis of specially formulated oligonucleotide tracers called
gene probes
33
A molecule labeled with a radioactive isotope, dye, or enzyme that is used to locate particular sequence or gene on a DNA molecule
DNA Probe
34
DNA probe is also known as
hybridization probe
35
hybridization probe is also known as
DNA Probe
36
A sequence that is used to locate a specific DNA/RNA fragment to take to PCR to be replicated.
DNA Probe
37
a technique that syntheysize large quantities of a DNA fragment without cloning it.
Polymerase Chain Reaction (PCR)
38
By this technique, large quantities of a particular DNA sequence can be prepared
Polymerase Chain Reaction (PCR)
39
This method can generate tens of billions of copies of a particular DNA fragment (the sequence of interest, DNA of interest, or target DNA) from a DNA extract (DNA template)
Polymerase Chain Reaction (PCR)
40
Three Major Steps Involved in the PCR Technique:
1) denaturation 2) annealing 3) extension
41
Steps Involved in the PCR Technique
STEP 1; the DNA is denatured at high temperatures(from 90 - 97 degrees Celsius). STEP 2: primers anneal to the DNA template strands to prime extension. STEP 3: extension occurs at the end of the annealed primers to create a complementary copy strand of DNA.
42
DNA is denatured in what temperatures?
90 - 97°C
43
This step in the PCR cycle effectively doubles the DNA quantity
STEP 3: Extension
44
Denaturation temperature
94°C
45
Annealing temperature
54°C
46
Extension temperature
72°C
47
A PCR can either be what?
Qualitative or Quantitative
48
is also referred to real time PCR
Quantitative PCR techniques
49
It gives an idea about how much DNA amount present in the sample
Quantitative PCR techniques
50
Is used for detecting a specific DNA segment
Qualitative PCR techniques
51
is another modification of PCR in which two or more target sequences can be demonstrated simultaneously in a single specimen at the same time
Multiplex PCR
52
is designed to collect data as the reaction is proceeding, which is more accurate for DNA and RNA quantitation and does not require laborious post PCR methods.
Real-time PCR
53
A technique mainly used to change the phenotype of an organism (host) when a genetically altered vector is introduced and integrated into the genome of the organism
Recombinant DNA Technology
54
Basically, this process introduce a foreign piece of DNA structure into the genome of the host. This particular gene that is introduced is referred to as the recombinant gene.
Recombinant DNA Technology
55
This particular gene that is introduced in Recombinant DNA Technology is referred to as the
recombinant gene
56
Biological Products of Recombinant DNA Technology
Genetically Modified Organisms (GMOs) Gene Therapy
57
The process of artificially introducing foreign genes into organisms is termed
transfection
58
are available for a variety of biotechnological applications. Because they are unique life forms that would never have otherwise occurred, they can be patented.
Transgenic “designer” organisms
59
is a technique for replacing a faulty gene with a normal one in individuals with fatal or extremely debilitating genetic diseases.
Gene therapy
60
The inherent benefit of this therapy is to permanently cure the physiological dysfunction by repairing the genetic defect
Gene therapy