Chapter 3 Flashcards
Cell
Living structural and functional units enclosed by a membrane
Cell Division
One cell divides into 2 identical cells
Cell Biology/cytology
Is the study of cellular structure and function
Plasma Membrane
Forms the cell’s flexible outer surface separating the cells internal/external environment
Internal environment
Everything inside the cell
External environment
Everything outside the cell
Cytoplasm
Consists of all the cellular contents between the plasma membrane and the nucleus.
What compartments does Cytoplasm consist of
Cytosol and Organelles
Cytosol
fluid portion of cytoplasm
Organelles
Includes cytoskeleton, ribosomes, endoplasmic reticulum, Golgi complex, lysosomes, peroxisomes and mitochondria. Each have specific functions, shape, characteristics
Nucleus
a large organelle that houses most of a cell’s DNA
Chromosomes
A single molecule of DNA associated with several proteins
Genes
Hereditary units that control most aspects of cellular structure and function
Plasma Membrane
Flexible yet sturdy barrier that surrounds and contains cytoplasm
What Structural Model does the Plasma Membrane use and describe it
Fluid mosaic model- the molecular arrangement of plasma membrane resembles a continually moving sea of fluid lipids that contains a mosaic of many different proteins.
Basic Structural framework of the Plasma Membrane
Lipid Bilayer
Lipid Bilayer
2 back to back layers made up of 3 types of lipid molecules
3 types of lipid molecules in the lipid bilayer
Phospholipids (75%, contain phosphorus), Cholesterol (20%, a steroid with an attached OH), Glycolipid (5%, lipid with an attached carbohydrate)
Amphipathic
molecules that have both polar and non-polar parts
Membrane Proteins
Integral or peripheral according to whether they are firmly embedded in the membrane
Integral Protein
Extend into or through the lipid bilayer and are firmly embedded into it
Transmembrane protein
Usually an integral protein. This means that they span the entire lipid bilayer and protrude into both the cytosol and extracellular fluid
Peripheral Protein
Not as firmly embedded in the membrane. They are attached to the polar heads of membrane lipids or to integral proteins at the inner/outer surface of the membrane
Glycoprotein
proteins with carbohydrate groups attached to the ends that protrude into the extracellular fluid
Glycocalyx
the carbohydrate portions of glycolipids and glycoproteins form an extensive sugary coating
4 Functions of Plasma Proteins
1.Act as barrier separating inside and outside of wall
2.Controls the flow of substances in and out of the cell.
3.Helps identify the cell to other cells
4.Participates in intracellular signalling
Membrane Protein Functions
Ion Channels, Carriers, Receptors, Enzymes, Linkers, Cell-identity markers
Ion Channels
Pores/holes that specific ions, such as K+ can flow through to get into/out of the cell
Carriers
Selectively moving a polar substance or ion from 1 side of the membrane to the other; also known as transporters
Receptors
Serve as cellular recognition sites; each type of receptor recognizes and binds a specific type of molecule
Ligand
a specific molecule that binds to a receptor
Enzymes
That catalyze specific chemical reactions at the inside/outside surface of the cell
Linkers
the anchor proteins into the plasma membranes of neighbouring cells to 1 another or to a protein filaments inside/outside the cell; peripheral proteins serve as enzymes/linkers
Cell-Identity Markers
May enable a cell to recognize other cells or same kind during tissue formation/recognize and respond to potentially dangerous foreign cells
Membrane Permeability-Permeable
Permeable means a structure permits passage of substances through it
Membrane Permeability-Impermeable
means a structure does not permit the passage of substances through it
Selective Permeability
Plasma membranes permit some substances to pass more readily than others through it
Concentration Gradient
Is the difference in the concentration of a chemical from one place to another (such as fro inside a cell to outside of a plasma membrane)
Electrical Gradient
Typically the inner surface of the plasma membrane is more negatively charged and then outer surface is more positively charged; a difference in the electrical charges between 2 regions
Membrane potential
the charge difference, because the electrical gradient occurs across the plasma membrane
Electrochemical Gradient
the combined influence of the concentration gradient and the electrical gradient on movement of a particular ion
What is essential to the life of a cell
transport across the plasma membrane
Passive Processes
when a substance moves down its concentration or electrical gradient to cross membrane using only its own kinetic energy/kinetic energy intrinsic to the particles moving/no input energy from the cell (diffusion)
Active Processes
Cellular energy used to drive the substance ‘uphill’ against its concentration or electrical gradient-usually in the form of ATP
Vesicles
another way substances may enter/leave cells- an active process in which tiny spherical membrane sacs are used- Active transport
Diffusion
is a passive process in which the random mixing of particles in a solution occurs because of the particles’ kinetic energy
Solutes and Solvents
Both undergo diffusion
Factors that influence diffusion rate
Steepness of concentration gradient, temperature, mass of diffusing substance, surface area, diffusion distance,
Simple Diffusion
a passive process in which substances move freely through the lipid bilayer of the plasma membranes of cells w/o the help of membrane transport proteins
Facilitated diffusion
solutes are too polar or highly charged to move through the lipid bilayer by simple diffusion can cross the plasma membrane by passive process
Channel-mediated facilitated diffusion
a solute moves down its concentration gradient across the lipid bilayer through a membrane channel
Ion Channels
Integral transmembrane proteins that allow passage of small inorganic ions that are too hydrophilic to penetrate the nonpolar layer of the lipid bilayer
Gated Channel
When part of the channel protein acts as a plug/gate, changing shape in one way to open pore and in another way to close it
Carrier/Transport-mediated facilitated diffusion
Moves a solute down its concentration gradient across the plasma membrane
Transport Maximum
the # of carriers available in a plasma membrane places an upper limit, on the rate in which facilitated diffusion can occur
Osmosis
A type of diffusion in which there is net movement of a solvent through a selectively permeable membrane
Aquaporins
Integral membrane protein that functions as water channels
Hydrostatic pressure
Forces water molecules to move back into the left arm
Osmotic Pressure
The solution with an impermeable solute also exerts a force, the pressure of a solution is proportional to the concentration of the solute particles that can’t cross the membrane–>higher solute concentration, higher solute’s osmotic pressure
Tonicity
Is a measure of the solutions ability to change the volume of cells by altering their water content
Isotonic Solution
Any solution in which a cell—for example, a red blood cell (RBC)—
maintains its normal shape and volume
Hypotonic Solution
A solution that has a lower concentration of solutes than the cytosol inside the RBCs
Hemolysis
To split apart RBC’s
Lysis
the rupture of other types of
cells due to placement in a hypotonic solution
Hypertonic Solution
has a higher concentration of solutes than does the cytosol inside
RBCs
Crenation
In a solution water molecules move out of the cells
faster than they enter, causing the cells to shrink. The shrinkage is called crenation
Active Transport
an active process because energy is required for carrier
proteins to move solutes across the membrane against a concentration
gradient. Two sources of cellular energy can be used to drive active
transport: (1) Energy obtained from hydrolysis of adenosine triphosphate
(ATP) is the source in primary active transport; (2) energy stored in an ionic
concentration gradient is the source in secondary active transport.
Primary Active Transport
energy
derived from hydrolysis of ATP changes the shape of a carrier protein,
which “pumps” a substance across a plasma membrane against its concentration gradient.
Pumps
carrier proteins that mediate primary active
transport
Na+/K+ Pump or Na+/K+ ATPase
The most prevalent primary active transport mechanism expels
sodium ions (Na+) from cells and brings potassium ions (K+) in. Because of the specific ions it moves, this carrier is called the sodium–
potassium pump. Because a part of the sodium–potassium pump
acts as an ATPase, an enzyme that hydrolyzes ATP, another name for
this pump is Na+–K+ ATPase.
Secondary Active Transport
the
energy stored in a Na+ or H+ concentration gradient is used to drive
other substances across the membrane against their own concentration gradients. Because a Na+ or H+ gradient is established by primary active transport, secondary active tr
Symporters
Move 2 substances in the same direction
Antiporters
move 2 substance in opposite directions across the membrane
Transport in vesicles
transport a variety of substances from one structure to another w/n cells
Vesicles
A small spherical sac
Exocytosis
Materials move out of a cell by the fusion with plasma membrane of vesicles formed inside the cell
Endocytosis
Materials move into a cell in a vesicle forms from the plasma membrane
Receptor Mediated Endocytosis
is a highly selective type of endocytosis by which cells take up specific ligands